| Multitasking by multivalent circular DNA aptamers. | |
| | |
MedLine Citation:
|
PMID: 16482500 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Nucleic acid aptamers are finding increasing applications in biology, especially as therapeutic candidates and diagnostic components. An important characteristic in meeting the needs of these applications is improved stability in physiological fluids, which is most often accomplished with chemical modification or unnatural nucleotides. In an alternative approach we have specified the design of a multivalent circular DNA aptamer topology that encompasses a number of properties relevant to nucleic acid therapeutic candidates, especially the ability to multitask by combining different activities together within a modular structure. Improved stability in blood products, greater conformational stability, antidoting by complementary circular antiaptamers, heterovalency, transcription factor decoy activity and minimal unintended effects upon the cellular innate immune response are desirable properties that are described here. Multitasking by circular DNA aptamers could similarly find applications in diagnostics and biomaterials, where the combination of interchangeable modules might generate new functions, such as anticoagulation coupled with reversible cell capture as, described here. These results provide a platform for further exploration of multivalent circular aptamer properties, especially in novel combinations of nucleic acid therapeutic modes. |
| | |
Authors:
|
Daniel A Di Giusto; Sarah M Knox; Yuching Lai; Gregory D Tyrelle; May T Aung; Garry C King |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Chembiochem : a European journal of chemical biology Volume: 7 ISSN: 1439-4227 ISO Abbreviation: Chembiochem Publication Date: 2006 Mar |
Date Detail:
|
Created Date: 2006-03-08 Completed Date: 2006-06-13 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 100937360 Medline TA: Chembiochem Country: Germany |
Other Details:
|
Languages: eng Pagination: 535-44 Citation Subset: IM |
Affiliation:
|
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia. daniel@kinglab.unsw.edu.au |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aptamers, Nucleotide
/
chemistry* Cell Separation Cells, Cultured DNA, Circular / chemistry* Flow Cytometry Humans |
| Chemical | |
Reg. No./Substance:
|
0/Aptamers, Nucleotide; 0/DNA, Circular |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cyclin D3 expression in primary Ta/T1 bladder cancer.
Next Document: Phospholipases and galactolipases trigger oxylipin-mediated wound-activated defence in the red alga ...