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Multistep enzyme catalyzed reactions for unnatural amino acids.
MedLine Citation:
PMID:  21956554     Owner:  NLM     Status:  In-Data-Review    
The use of unnatural amino acids, particularly synthetic α-amino acids, for modern drug discovery research requires the availability of enantiomerically pure isomers. Starting from a racemate, one single enantiomer can be obtained using a deracemization process. The two more common strategies of deracemization are those obtained by stereoinversion and by dynamic kinetic resolution. Both techniques will be here described using as a substrate the D: ,L: -3-(2-naphthyl)-alanine, a non-natural amino acid: the first one employing a multi-enzymatic redox system, the second one combining an hydrolytic enzyme together with a base-catalyzed substrate racemization. In both cases, the final product, L: -3-(2-naphthyl)alanine, is recovered with good yield and excellent enantiomeric excess.
Paola D'Arrigo; Davide Tessaro
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  794     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-09-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  21-35     Citation Subset:  IM    
Dipartimento di Chimica, Materiali ed Ingegneria Chimica "Giulio Natta" Politecnico di Milano, and "The Protein Factory", Politecnico di Milano and Università degli Studi dell'Insubria, Milano, Italy,
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