Document Detail


Multiscale iterative voting for differential analysis of stress response for 2D and 3D cell culture models.
MedLine Citation:
PMID:  21118235     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Three-dimensional (2D) cell culture models have emerged as the basis for improved cell systems biology. However, there is a gap in robust computational techniques for segmentation of these model systems that are imaged through confocal or deconvolution microscopy. The main issues are the volume of data, overlapping subcellular compartments and variation in scale or size of subcompartments of interest, which lead to ambiguities for quantitative analysis on a cell-by-cell basis. We address these ambiguities through a series of geometric operations that constrain the problem through iterative voting and decomposition strategies. The main contributions of this paper are to (i) extend the previously developed 2D radial voting to an efficient 3D implementation, (ii) demonstrate application of iterative radial voting at multiple subcellular and molecular scales, and (iii) investigate application of the proposed technology to two endpoints between 2D and 3D cell culture models. These endpoints correspond to kinetics of DNA damage repair as measured by phosphorylation of γH2AX, and the loss of the membrane-bound E-cadherin protein as a result of ionizing radiation. Preliminary results indicate little difference in the kinetics of the DNA damage protein between 2D and 3D cell culture models; however, differences between membrane-bound E-cadherin are more pronounced.
Authors:
J Han; H Chang; Q Yang; G Fontenay; T Groesser; M Helen Barcellos-Hoff; B Parvin
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Publication Detail:
Type:  Journal Article     Date:  2010-08-26
Journal Detail:
Title:  Journal of microscopy     Volume:  241     ISSN:  1365-2818     ISO Abbreviation:  J Microsc     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  England    
Other Details:
Languages:  eng     Pagination:  315-26     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors Journal of Microscopy © 2010 The Royal Microscopical Society.
Affiliation:
Lawrence Berkeley National Laboratory, Berkeley, CA, U.S.A.
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