Document Detail


Multiprotein complexes present at the MIF motifs flanking the promoter of the human c-myc gene.
MedLine Citation:
PMID:  10828444     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The activated c-myc allele in Burkitt's lymphoma is associated with a clustering of somatic mutations within a discrete domain of intron I that define protein recognition sequences, designated as myc intron factors (MIF-1, MIF-2 and MIF-3). We have previously shown that MIF-1 binding activity consists of two polypeptides, myc intron binding polypeptide (MIBP1) and RFX1. In the present study we identified two polypeptides, p105 and p115, and showed that these proteins give rise to a DNA-protein complex at the MIF-2 as well as the adjacent MIF-1 site. In addition, we demonstrated that all four proteins interact with a novel MIF-1 like motif upstream from the c-myc promoter region, designated 5'MIF. These data suggest a model, where the interactions of MIBP1/RFX1 and p105/p115 with the MIF-like sites may play a role in the promoter topology of the c-myc gene.
Authors:
A Itkes; C J Allegra; M Zajac-Kaye
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  474     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-06     Completed Date:  2000-07-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  23-8     Citation Subset:  IM    
Affiliation:
Department of Developmental Therapeutics, Medicine Branch, Division of Clinical Sciences, National Cancer Institute, Navy Oncology Branch, Building 8, R 5101, Naval Hospital, 20889, Bethesda, MD, USA.
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MeSH Terms
Descriptor/Qualifier:
Burkitt Lymphoma / genetics*
DNA / metabolism
DNA-Binding Proteins / metabolism*
Enhancer Elements, Genetic
Genes, myc / genetics*
Hela Cells
Humans
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid
Repressor Proteins
Transcription Factors / metabolism*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Repressor Proteins; 0/Transcription Factors; 0/regulatory factor X transcription factors; 148770-78-9/HIVEP2 protein, human; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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