Document Detail

Multipotency of a bone marrow stromal cell line, TBR31-2, established from ts-SV40 T antigen gene transgenic mice.
MedLine Citation:
PMID:  10679225     Owner:  NLM     Status:  MEDLINE    
Bone marrow is believed to contain multipotential stromal stem cells which can differentiate into osteoblasts, chondrocytes, adipocytes, and myoblasts (Prockop, D. J. Science 276, 71-74, 1997). Therefore, characterization and identification of the stem-like cell within the stromal cells are important to understand bone marrow function in relation to the hematopoietic microenvironment, and repair/regeneration of tissue defects. TBR31-2 cell, a bone marrow stromal cell line established from bone marrow of transgenic mice harboring temperature-sensitive (ts) simian virus (SV) 40T-antigen gene for immortality, is induced toward both adipocytic and osteogenic cells under conditions of the inactivation of T-antigen (Okuyama, R., Yanai, N., Obinata, M. Exp. Cell Res. 218, 424-429, 1995). In this work, using a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, mRNA expressions of tissue-specific differentiation markers for adipocyte (lipoprotein lipase), osteoblast (type I collagen and osteocalcin), chondrocyte (type II and X collagen), and muscle cell (desmin) were examined during a long-term culture of the cell. In addition, histochemical studies showed the appearance of adipocytic, osteoblastic, chondrocytic, and muscle cells during this long-term culture. Thus, TBR31-2, which has characteristics of an undifferentiated cell, has the potential to express the multipotential cell lineages. These results indicated that a multipotential progenitor cell including potential to differentiate into a muscle cell and which is situated in the mesenchymal cell lineage was first obtained.
Y Negishi; A Kudo; A Obinata; K Kawashima; H Hirano; N Yanai; M Obinata; H Endo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  268     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-10     Completed Date:  2000-03-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  450-5     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa, 199-0195, Japan.
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MeSH Terms
Antigens, Differentiation / biosynthesis,  genetics
Antigens, Polyomavirus Transforming / genetics*
Bone Marrow Cells / cytology*,  metabolism,  ultrastructure
Cell Differentiation*
Cell Line / cytology*
Cell Size
Gene Expression
Mice, Transgenic
Microscopy, Electron
Stromal Cells / cytology,  metabolism,  ultrastructure
Reg. No./Substance:
0/Antigens, Differentiation; 0/Antigens, Polyomavirus Transforming

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