Document Detail


Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors.
MedLine Citation:
PMID:  23138230     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells rely on a complex, interconnected network of signaling pathways to sense and interpret changes in their extracellular environment. The development of genetically encoded fluorescent protein (FP)-based biosensors has made it possible for researchers to directly observe and characterize the spatiotemporal dynamics of these intracellular signaling pathways in living cells. However, detailed information regarding the precise temporal and spatial relationships between intersecting pathways is often lost when individual signaling events are monitored in isolation. As the development of biosensor technology continues to advance, it is becoming increasingly feasible to image multiple FP-based biosensors concurrently, permitting greater insights into the intricate coordination of intracellular signaling networks by enabling parallel monitoring of distinct signaling events within the same cell. In this review, we discuss several strategies for multiplexed imaging of FP-based biosensors, while also underscoring some of the challenges associated with these techniques and highlighting additional avenues that could lead to further improvements in parallel monitoring of intracellular signaling events.
Authors:
Charlene Depry; Sohum Mehta; Jin Zhang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2012-11-09
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  465     ISSN:  1432-2013     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-27     Completed Date:  2013-08-13     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  373-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques
Fluorescence Resonance Energy Transfer
Fluorescent Dyes*
Green Fluorescent Proteins / genetics*,  metabolism
Humans
Microscopy, Fluorescence / methods*
Optical Imaging / methods
Optogenetics*
Proteins / genetics,  metabolism
Signal Transduction*
Grant Support
ID/Acronym/Agency:
DP1 CA174423/CA/NCI NIH HHS; DP1CA174423/CA/NCI NIH HHS; R01 DK073368/DK/NIDDK NIH HHS; R01DK073368/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Proteins; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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