Document Detail


Multiplexed profiling of secreted proteins for the detection of potential space biomarkers.
MedLine Citation:
PMID:  21461557     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Space travel exposes astronauts to a plethora of potentially detrimental conditions, such as cosmic radiation and microgravity. As both factors are hard to simulate on Earth, present knowledge remains limited. However, this knowledge is of vital importance, making space flight experiments a necessity for determining the biological effects and the underlying biochemical processes, especially when keeping future long-term interplanetary missions in mind. Instead of estimating the long-term effects, which usually implicate severe endpoints (e.g., cancer) and which are often difficult to attribute, research has shifted to finding representative biomarkers for rapid and sensitive detection of individual radiosensitivity. In this context, an appealing set of candidate markers is the group of secreted proteins, as they exert an intercellular signaling function and are easy to assess. We screened a subset of secreted proteins in cells exposed to space travel by means of multiplex bead array analysis. To determine the cell-specific signatures of the secreted molecules, we compared the conditioned medium of normal fibroblast cells to fibroblasts isolated from a patient with Hutchinson-Gilford Progeria syndrome, which are known to have a perturbed nuclear architecture and DNA damage response. Out of the 88 molecules screened, 20 showed a significant level increase or decrease, with a differential response to space conditions between the two cell types. Among the molecules that were retained, which may prove to be valuable biomarkers, are apolipoprotein C-III, plasminogen activator inhibitor type 1, β-2-microglobulin, ferritin, MMP-3, TIMP-1 and VEGF.
Authors:
Birger Dieriks; Winnok H De Vos; Marjan Moreels; Myriam Ghardi; Raoul Hennekam; Jos L V Broers; Sarah Baatout; Patrick van Oostveldt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-30
Journal Detail:
Title:  Molecular medicine reports     Volume:  4     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:    2011 Jan-Feb
Date Detail:
Created Date:  2011-04-04     Completed Date:  2011-08-01     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  17-23     Citation Subset:  IM    
Affiliation:
Bioimaging and Cytometry Unit, Department of Molecular Biotechnology, Ghent University, 9000 Gent, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / metabolism
Cells, Cultured
Fibroblasts / metabolism*
Humans
Progeria / metabolism
Proteins / metabolism*
Radiation Tolerance
Space Flight*
Weightlessness
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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