Document Detail


Multiplexed colorimetric detection of Kaposi's sarcoma associated herpesvirus and Bartonella DNA using gold and silver nanoparticles.
MedLine Citation:
PMID:  23340972     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kaposi's sarcoma (KS) is an infectious cancer occurring most commonly in human immunodeficiency virus (HIV) positive patients and in endemic regions, such as Sub-Saharan Africa, where KS is among the top four most prevalent cancers. The cause of KS is the Kaposi's sarcoma-associated herpesvirus (KSHV, also called HHV-8), an oncogenic herpesvirus that while routinely diagnosed in developed nations, provides challenges to developing world medical providers and point-of-care detection. A major challenge in the diagnosis of KS is the existence of a number of other diseases with similar clinical presentation and histopathological features, requiring the detection of KSHV in a biopsy sample. In this work we develop an answer to this challenge by creating a multiplexed one-pot detection system for KSHV DNA and DNA from a frequently confounding disease, bacillary angiomatosis. Gold and silver nanoparticle aggregation reactions are tuned for each target and a multi-color change system is developed capable of detecting both targets down to levels between 1 nM and 2 nM. The system developed here could later be integrated with microfluidic sample processing to create a final device capable of solving the two major challenges in point-of-care KS detection.
Authors:
Matthew Mancuso; Li Jiang; Ethel Cesarman; David Erickson
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Nanoscale     Volume:  5     ISSN:  2040-3372     ISO Abbreviation:  Nanoscale     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-04     Completed Date:  2013-07-16     Revised Date:  2014-03-28    
Medline Journal Info:
Nlm Unique ID:  101525249     Medline TA:  Nanoscale     Country:  England    
Other Details:
Languages:  eng     Pagination:  1678-86     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bartonella / genetics*,  isolation & purification
Colorimetry / methods*
DNA, Bacterial / analysis,  genetics*
DNA, Viral / analysis,  genetics*
Gold / diagnostic use
Herpesvirus 8, Human / genetics*,  isolation & purification
Metal Nanoparticles / diagnostic use*
Silver / diagnostic use
Grant Support
ID/Acronym/Agency:
UL1 RR 024996/RR/NCRR NIH HHS; UL1 RR024996/RR/NCRR NIH HHS; UL1 TR000457/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Viral; 3M4G523W1G/Silver; 7440-57-5/Gold
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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