Document Detail


Multiplex real-time PCR detection of heat-labile and heat-stable toxin genes in enterotoxigenic Escherichia coli.
MedLine Citation:
PMID:  16496584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A multiplex real-time PCR method was developed for detection of heat-labile and heat-stable toxin genes in enterotoxigenic Escherichia coli. Approximately 10 CFU per reaction mixture could be detected in rinsates from produce samples. Several foods representative of varieties previously shown to have caused enterotoxigenic E. coli outbreaks were spiked and enriched for 4 or 6 h. Both heat-labile and heat-stable toxin genes could be detected in the foods tested, with the exception of hot sauce, with threshold cycle values ranging from 25.2 to 41.1. A procedure using membrane filtration which would allow enumeration of the enterotoxigenic E. coli population in a food sample in less than 28 h by real-time PCR analysis of colonies picked from media highly selective for E. coli was also developed.
Authors:
Michael A Grant; Jinxin Hu; Karen C Jinneman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  69     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-24     Completed Date:  2006-03-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  412-6     Citation Subset:  IM    
Affiliation:
Pacific Regional Laboratory Northwest, US Food and Drug Administration, Bothell, Washington 98021, USA. mike.grant@fda.gov
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Bacterial Toxins / genetics*
Colony Count, Microbial
DNA Primers
Enterotoxins / genetics*
Escherichia coli / isolation & purification*
Food Contamination / analysis*
Hot Temperature*
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/DNA Primers; 0/Enterotoxins; 0/heat stable toxin (E coli)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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