Document Detail


Multiplex measurement of cytokine/receptor gene polymorphisms and interaction between interleukin-10 (-1082) genotype and chorioamnionitis in extreme preterm delivery.
MedLine Citation:
PMID:  16814164     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To establish a multiplex amplification refractory mutation system (ARMS) in fluid and dried whole blood, and to perform a pilot study to examine the role for single-nucleotide polymorphisms (SNPs) of inflammation-associated genes (interleukin [IL]-1 and -10, tumor necrosis factor-alpha [TNFA], and toll-like receptor-4 [TLR4]) and their interaction with clinical chorioamnionitis (CAM) in prematurity. METHODS: We established a quadruplex ARMS to detect the four above SNPs. Fifty-four women delivered at gestational age less than 32 weeks and 83 healthy female volunteers were genotyped. We compared (1) mothers of preterm infants with volunteers, and (2) women delivered before 29 weeks' gestation (n = 29) with those delivered at 29 to 31 completed weeks (n = 25). RESULTS: Multiplex ARMS is feasible using both fluid and dried whole blood. We found no overall differences in genotype and allele frequencies between mothers of preterm infants and volunteers. Among women who had a preterm delivery, those with both CAM and IL10(-1082)*G allele, the risk for delivery before 29 weeks was markedly increased (odds ratio [OR] 22, 95% confidence interval [CI] 2.5 - 191). CONCLUSION: The presence of both CAM and IL10(-1082)*G might play a role in extreme preterm delivery less than 29 weeks.
Authors:
Julia Kerk; Michael Dördelmann; Dorothee B Bartels; Maria-Jantje Brinkhaus; Christiane E L Dammann; Thilo Dörk; Olaf Dammann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the Society for Gynecologic Investigation     Volume:  13     ISSN:  1556-7117     ISO Abbreviation:  J. Soc. Gynecol. Investig.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-03     Completed Date:  2006-08-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9433806     Medline TA:  J Soc Gynecol Investig     Country:  United States    
Other Details:
Languages:  eng     Pagination:  350-6     Citation Subset:  IM    
Affiliation:
Department of Gynecology and Obstetrics, Hannover Medical School, Hannover, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chorioamnionitis / genetics*
Cytokines / genetics*
DNA / blood
Female
Genotype
Gestational Age
HELLP Syndrome / genetics
Humans
Infant, Newborn
Interleukin-1 / genetics
Interleukin-10 / genetics*
Obstetric Labor, Premature / genetics*
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide*
Pregnancy
Pregnancy, Multiple
Toll-Like Receptor 4 / genetics*
Tumor Necrosis Factor-alpha / genetics
Grant Support
ID/Acronym/Agency:
HL 04436/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 0/Interleukin-1; 0/TLR4 protein, human; 0/Toll-Like Receptor 4; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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