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A Multiplex Polymerase Chain Reaction Method for the Simultaneous Detection of GSTM1, GSTT1, and GSTP1 Polymorphisms.
MedLine Citation:
PMID:  24527777     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background: Glutathione S-transferases (GSTs) play an important role in the detoxification of a wide variety of toxicants. Among GSTs, GSTM1 and GSTT1 polymorphic deletions and the GSTP1 Ile105Val polymorphism were often studied in combination in many diseases because of their additive effects, but they were usually genotyped by two separate methods. Aim: The purpose of the present study was to develop a simple and reliable method to simultaneously detect these three polymorphisms. Methods: The three polymorphisms of 259 volunteers were genotyped using a multiplex polymerase chain reaction (PCR) method based on a tetra-primer amplification refractory mutation system-PCR (T-ARMS-PCR), and the results were validated by multiplex PCR for GSTM1 and GSTT1 polymorphisms and DNA sequencing of the GSTP1 Ile105Val polymorphism, respectively. Results: The multiplex PCR method of GSTM1, GSTT1, and GSTP1 polymorphisms based on T-ARMS-PCR can simultaneous detect the three polymorphisms in a single PCR. The results of this method are in perfect accord with the results of the multiplex PCR method of determining GSTM1, GSTT1 polymorphisms, and DNA sequencing of GSTP1 Ile105Val polymorphism. Conclusion: The novel multiplex PCR method of GSTM1, GSTT1, and GSTP1 polymorphisms is simple, fast, low-cost, and reliable for the simultaneous detection of GSTM1, GSTT1, and GSTP1 Ile105Val polymorphisms.
Authors:
Yan Deng; Wei Cai; Jixiong Xu; Weifeng Zhu; Daya Luo; Wei Zhang; Weiwei Liu; Fusheng Wan; Jinglin Yi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-2-14
Journal Detail:
Title:  Genetic testing and molecular biomarkers     Volume:  -     ISSN:  1945-0257     ISO Abbreviation:  Genet Test Mol Biomarkers     Publication Date:  2014 Feb 
Date Detail:
Created Date:  2014-2-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101494210     Medline TA:  Genet Test Mol Biomarkers     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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