| Multiplex MassTag-PCR for respiratory pathogens in pediatric nasopharyngeal washes negative by conventional diagnostic testing shows a high prevalence of viruses belonging to a newly recognized rhinovirus clade. | |
| | |
MedLine Citation:
|
PMID: 18674964 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Respiratory infections are the most common infectious diseases in humans worldwide and are a leading cause of death in children less than 5 years of age. OBJECTIVES: Identify candidate pathogens in pediatric patients with unexplained respiratory disease. STUDY DESIGN: Forty-four nasopharyngeal washes collected during the 2004-2005 winter season from pediatric patients with respiratory illnesses that tested negative for 7 common respiratory pathogens by culture and direct immunofluorescence assays were analyzed by MassTag-PCR. To distinguish human enteroviruses (HEV) and rhinoviruses (HRV), samples positive for picornaviruses were further characterized by sequence analysis. RESULTS: Candidate pathogens were detected by MassTag PCR in 27 of the 44 (61%) specimens that previously were rated negative. Sixteen of these 27 specimens (59%) contained picornaviruses; of these 9 (57%) contained RNA of a recently discovered clade of rhinoviruses. Bocaviruses were detected in three patients by RT-PCR. CONCLUSIONS: Our study confirms that multiplex MassTag-PCR enhances the detection of pathogens in clinical specimens, and shows that previously unrecognized rhinoviruses, that potentially form a species HRV-C, may cause a significant amount of pediatric respiratory disease. |
| | |
Authors:
|
Samuel R Dominguez; Thomas Briese; Gustavo Palacios; Jeffrey Hui; Joseph Villari; Vishal Kapoor; Rafal Tokarz; Mary P Glodé; Marsha S Anderson; Christine C Robinson; Kathryn V Holmes; W Ian Lipkin |
Related Documents
:
|
6669974 - Housing standards, social group, and respiratory infections in children of upernavik, g... 11827904 - Hypoxaemia in acute respiratory and non-respiratory illnesses in neonates and children ... 18448964 - Recently identified factors predisposing children to infectious diseases. 16102654 - Infection control, hospital epidemiology, and patient safety. 15526044 - Assessment of volume depletion in children with malaria. 8275214 - Gender and age differentials in risk factors for childhood malnutrition in bangladesh. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology Volume: 43 ISSN: 1386-6532 ISO Abbreviation: J. Clin. Virol. Publication Date: 2008 Oct |
Date Detail:
|
Created Date: 2008-09-10 Completed Date: 2008-12-01 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 9815671 Medline TA: J Clin Virol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 219-22 Citation Subset: IM |
Affiliation:
|
Department of Pediatrics, The Children's Hospital, University of Colorado Denver School of Medicine, Aurora, CO, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acute Disease Child, Preschool Enterovirus / isolation & purification Enterovirus Infections / epidemiology, virology Female Humans Infant Male Nasopharynx / virology* Picornaviridae Infections / epidemiology, virology* Polymerase Chain Reaction / methods Prevalence Respiratory Tract Infections* / epidemiology, virology Rhinovirus* / classification, isolation & purification Virus Diseases* / epidemiology, virology |
| Grant Support | |
ID/Acronym/Agency:
|
P01 AI059576-01/AI/NIAID NIH HHS; P01 AI059576-010001/AI/NIAID NIH HHS; P01 AI059576-02/AI/NIAID NIH HHS; P01 AI059576-020001/AI/NIAID NIH HHS; P01 AI059576-03/AI/NIAID NIH HHS; P01 AI059576-030001/AI/NIAID NIH HHS; P01 AI059576-04/AI/NIAID NIH HHS; P01 AI059576-040001/AI/NIAID NIH HHS; P01 AI059576-05/AI/NIAID NIH HHS; P01 AI059576-050001/AI/NIAID NIH HHS; P01-AI-059576/AI/NIAID NIH HHS; U01 AI070411-01/AI/NIAID NIH HHS; U01 AI070411-02/AI/NIAID NIH HHS; U01 AI070411-03/AI/NIAID NIH HHS; U01AI070411/AI/NIAID NIH HHS; U54 AI057158-01/AI/NIAID NIH HHS; U54 AI057158-02/AI/NIAID NIH HHS; U54 AI057158-03/AI/NIAID NIH HHS; U54 AI057158-04/AI/NIAID NIH HHS; U54 AI057158-05/AI/NIAID NIH HHS; U54 AI057158-05S1/AI/NIAID NIH HHS; U54AI57158/AI/NIAID NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A cross-case analysis of technology-in-use practices: EPR-adaptation in Canada and Norway.
Next Document: One-step RT-PCR for detection of Zika virus.