Document Detail


Multiple transcriptional domains, with distinct left and right components, in the atrial chambers of the developing heart.
MedLine Citation:
PMID:  11090542     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During heart development, 2 fast-conducting regions of working myocardium balloon out from the slow-conducting primary myocardium of the tubular heart. Three regions of primary myocardium persist: the outflow tract, atrioventricular canal, and inflow tract, which are contiguous throughout the inner curvature of the heart. The contribution of the inflow tract to the definitive atrial chambers has remained enigmatic largely because of the lack of molecular markers that permit unambiguous identification of this myocardial domain. We now report that the genes encoding atrial natriuretic factor, myosin light chain (MLC) 3F, MLC2V, and Pitx-2, and transgenic mouse lines expressing nlacZ under the control of regulatory sequences of the mouse MLC1F/3F gene, display regionalized patterns of expression in the atrial component of the developing mouse heart. These data distinguish 4 broad transcriptional domains in the atrial myocardium: (1) the atrioventricular canal that will form the smooth-walled lower atrial rim proximal to the ventricles; (2) the atrial appendages; (3) the caval vein myocardium (systemic inlet); and (4) the mediastinal myocardium (pulmonary inlet), including the atrial septa. The pattern of expression of Pitx-2 reveals that each of these transcriptional domains has a distinct left and right component. This study reveals for the first time differential gene expression in the systemic and pulmonary inlets, which is not shared by the contiguous atrial appendages and provides evidence for multiple molecular compartments within the atrial chambers. Furthermore, this work will allow the contribution of each of these myocardial components to be studied in congenitally malformed hearts, such as those with abnormal venous return.
Authors:
D Franco; M Campione; R Kelly; P S Zammit; M Buckingham; W H Lamers; A F Moorman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Circulation research     Volume:  87     ISSN:  1524-4571     ISO Abbreviation:  Circ. Res.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-01     Completed Date:  2000-12-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  984-91     Citation Subset:  IM    
Affiliation:
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands. dfranco@ujaen.es
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aging / metabolism
Animals
Antigens, Differentiation / biosynthesis
Atrial Natriuretic Factor / genetics,  metabolism
Gene Expression Regulation, Developmental*
Genes, Reporter / genetics
Heart Atria / cytology,  embryology*
Homeodomain Proteins / genetics,  metabolism
Lac Operon
Mice
Mice, Inbred C57BL
Mice, Transgenic
Myocardium / cytology,  metabolism*
Myosin Light Chains / genetics,  metabolism
Nuclear Proteins*
Organ Specificity / genetics
Paired Box Transcription Factors
Rats
Rats, Wistar
Transcription Factors / genetics,  metabolism
Transcription, Genetic*
Transgenes
Chemical
Reg. No./Substance:
0/Antigens, Differentiation; 0/Homeodomain Proteins; 0/Myosin Light Chains; 0/Nuclear Proteins; 0/Paired Box Transcription Factors; 0/Transcription Factors; 0/homeobox protein PITX1; 0/homeobox protein PITX3; 184787-43-7/homeobox protein PITX2; 85637-73-6/Atrial Natriuretic Factor
Comments/Corrections
Comment In:
Circ Res. 2000 Nov 24;87(11):961-3   [PMID:  11090537 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The late phase of preconditioning.
Next Document:  Soluble epoxide hydrolase regulates hydrolysis of vasoactive epoxyeicosatrienoic acids.