Document Detail


Multiple signals are required for alpha2,6-sialyltransferase (ST6Gal I) oligomerization and Golgi localization.
MedLine Citation:
PMID:  15582997     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A single amino acid difference in the catalytic domain of two isoforms of the alpha2,6-sialyltransferase (ST6Gal I) leads to differences in their trafficking, processing, and oligomerization. The STtyr isoform is transiently localized in the Golgi and is ultimately cleaved and secreted, whereas the STcys isoform is stably localized in the Golgi and is not cleaved and secreted. The stable localization of STcys is correlated with its enhanced ability to oligomerize. To test the hypothesis that multiple signals can mediate Golgi localization and further evaluate the role of oligomerization in the localization process, we evaluated the effects of individually and simultaneously altering the cytosolic tail and transmembrane region of the STcys isoform. We found that the localization, processing, and oligomerization of STcys were not substantially changed when either the core amino acids of the cytosolic tail were deleted or the sequence and length of the transmembrane region were altered. In contrast, when these changes were made simultaneously, the STcys isoform was converted into a form that was processed, secreted, and weakly oligomerized like STtyr. We propose that STcys oligomerization is a secondary event resulting from its concentration in the Golgi via mechanisms independently mediated by its cytosolic tail and transmembrane region.
Authors:
Fiona H Fenteany; Karen J Colley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2004-12-06
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  280     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-14     Completed Date:  2005-04-18     Revised Date:  2013-03-22    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5423-9     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, College of Medicine, Chicago, IL 60607, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
COS Cells
Catalytic Domain
Golgi Apparatus / metabolism*
HeLa Cells
Humans
Hydrogen-Ion Concentration
Protein Isoforms / chemistry,  genetics,  metabolism
Protein Structure, Quaternary
Sequence Deletion / genetics
Sialyltransferases / chemistry*,  genetics,  metabolism*
Signal Transduction
Solubility
Grant Support
ID/Acronym/Agency:
R01 GM048134/GM/NIGMS NIH HHS; R01-GM48134/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Protein Isoforms; EC 2.4.99.-/Sialyltransferases; EC 2.4.99.1/beta-D-galactoside alpha 2-6-sialyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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