Document Detail

Multiple screening of urolithic organic acids with copper nanoparticle-plated electrode: potential assessment of urolithic risks.
MedLine Citation:
PMID:  19699709     Owner:  NLM     Status:  MEDLINE    
There is yet to be a reliable prediction of urolithiasis. To facilitate early diagnosis, a simple and rapid high performance liquid chromatography method with electrochemical detection using disposable copper-nanoparticle-plated electrodes (Cu(n)-SPE) was developed for multiple detection of creatinine and 4 urolithic organic acids. A total of 206 normal and urolithic human and canine urines and urolith samples were collected for direct analysis of creatinine, cystine, uric acid, oxalic acid, and citric acid without sample cleanup and derivatization processes. Urinary organic acids were separated in 11 min and were devoid of ascorbic acid interference. The detection limits (S/N>3) were at the nanomolar level with linear dynamic ranges spanning 2-3 orders of magnitude. Recoveries in urine ranged from 99.5% for creatinine to 86.5% for citric acid. The analytical variations (RSD) were less than 6.2% in phosphate buffer and 7.7% in urine. Important differences in organic acid levels/profiles between animal species and among normal and urolithic urines/urolith were unveiled and corresponded well (70-90%) with the urolithic risk in a retrospective assessment. The simplicity and reproducibility of this method using disposable Cu(n)-SPE has made routine urine analysis possible and can be of great clinical and diagnostic potential in the screening of urolithiasis and abnormal states related to excess secretion of organic acids and amino acids in humans and animals.
Chung-Wei Yang; Jyh-Myng Zen; Yu-Lin Kao; Cheng-Teng Hsu; Tung-Ching Chung; Chao-Chin Chang; Chi-Chung Chou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-21
Journal Detail:
Title:  Analytical biochemistry     Volume:  395     ISSN:  1096-0309     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-10-12     Completed Date:  2010-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  224-30     Citation Subset:  IM    
Veterinary Medical Teaching Hospital and Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan.
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MeSH Terms
Acids / urine*
Citric Acid / urine
Copper / chemistry*
Creatinine / urine
Cystine / analysis
Metal Nanoparticles / chemistry*
Oxalic Acid / urine
Uric Acid / urine
Urinalysis / methods*
Urolithiasis / diagnosis
Reg. No./Substance:
0/Acids; 144-62-7/Oxalic Acid; 56-89-3/Cystine; 60-27-5/Creatinine; 69-93-2/Uric Acid; 7440-50-8/Copper; 77-92-9/Citric Acid

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