| Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells. | |
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MedLine Citation:
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PMID: 18971378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To better understand the mechanism controlling nonmuscle myosin II (NM-II) assembly in mammalian cells, mutant NM-IIA constructs were created to allow tests in live cells of two widely studied models for filament assembly control. A GFP-NM-IIA construct lacking the RLC binding domain (DeltaIQ2) destabilizes the 10S sequestered monomer state and results in a severe defect in recycling monomers during spreading, and from the posterior to the leading edge during polarized migration. A GFP-NM-IIA construct lacking the nonhelical tailpiece (Deltatailpiece) is competent for leading edge assembly, but overassembles, suggesting defects in disassembly from lamellae subsequent to initial recruitment. The Deltatailpiece phenotype was recapitulated by a GFP-NM-IIA construct carrying a mutation in a mapped tailpiece phosphorylation site (S1943A), validating the importance of the tailpiece and tailpiece phosphorylation in normal lamellar myosin II assembly control. These results demonstrate that both the 6S/10S conformational change and the tailpiece contribute to the localization and assembly of myosin II in mammalian cells. This work furthermore offers cellular insights that help explain platelet and leukocyte defects associated with R1933-stop alleles of patients afflicted with human MYH9-related disorder. |
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Authors:
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Mark T Breckenridge; Natalya G Dulyaninova; Thomas T Egelhoff |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-10-29 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 20 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-01 Completed Date: 2009-03-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 338-47 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Amino Acid Sequence Animals Cell Movement / physiology* Cytoskeleton / metabolism Fluorescence Recovery After Photobleaching Hela Cells Humans Models, Molecular Mutagenesis Myosin Type II / chemistry, genetics, metabolism* Protein Conformation Recombinant Fusion Proteins / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DK007678/DK/NIDDK NIH HHS; GM 077224/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Recombinant Fusion Proteins; EC 3.6.1.-/Myosin Type II |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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