Document Detail


Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells.
MedLine Citation:
PMID:  18971378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To better understand the mechanism controlling nonmuscle myosin II (NM-II) assembly in mammalian cells, mutant NM-IIA constructs were created to allow tests in live cells of two widely studied models for filament assembly control. A GFP-NM-IIA construct lacking the RLC binding domain (DeltaIQ2) destabilizes the 10S sequestered monomer state and results in a severe defect in recycling monomers during spreading, and from the posterior to the leading edge during polarized migration. A GFP-NM-IIA construct lacking the nonhelical tailpiece (Deltatailpiece) is competent for leading edge assembly, but overassembles, suggesting defects in disassembly from lamellae subsequent to initial recruitment. The Deltatailpiece phenotype was recapitulated by a GFP-NM-IIA construct carrying a mutation in a mapped tailpiece phosphorylation site (S1943A), validating the importance of the tailpiece and tailpiece phosphorylation in normal lamellar myosin II assembly control. These results demonstrate that both the 6S/10S conformational change and the tailpiece contribute to the localization and assembly of myosin II in mammalian cells. This work furthermore offers cellular insights that help explain platelet and leukocyte defects associated with R1933-stop alleles of patients afflicted with human MYH9-related disorder.
Authors:
Mark T Breckenridge; Natalya G Dulyaninova; Thomas T Egelhoff
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-10-29
Journal Detail:
Title:  Molecular biology of the cell     Volume:  20     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-01     Completed Date:  2009-03-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  338-47     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Amino Acid Sequence
Animals
Cell Movement / physiology*
Cytoskeleton / metabolism
Fluorescence Recovery After Photobleaching
Hela Cells
Humans
Models, Molecular
Mutagenesis
Myosin Type II / chemistry,  genetics,  metabolism*
Protein Conformation
Recombinant Fusion Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
DK007678/DK/NIDDK NIH HHS; GM 077224/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Recombinant Fusion Proteins; EC 3.6.1.-/Myosin Type II
Comments/Corrections

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