| Multiple regulators control expression of the Entner-Doudoroff aldolase (Eda) of Escherichia coli. | |
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MedLine Citation:
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PMID: 15659677 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Escherichia coli eda gene, which encodes the Entner-Doudoroff aldolase, is central to the catabolism of several sugar acids. Here, we show that Eda synthesis is induced by growth on gluconate, glucuronate, or methyl-beta-D-glucuronide; phosphate limitation; and carbon starvation. Transcription of eda initiates from three promoters, designated P1, P2, and P4, each of which is responsible for induction under different growth conditions. P1 controls eda induction on gluconate and is regulated by GntR. P2 controls eda induction on glucuronate and galacturonate and is regulated by KdgR. P4 is active under conditions of phosphate starvation and is directly controlled by PhoB. In addition, CsrA activates Eda synthesis, apparently by an indirect mechanism that may be involved in the modest changes in expression level that are associated with carbon starvation. The complex regulation of eda is discussed with respect to its several physiological roles, which apparently accommodate not only sugar acid catabolism but also detoxification of metabolites that could accumulate during starvation-induced stress. |
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Authors:
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Elizabeth L Murray; Tyrrell Conway |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 187 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-01-20 Completed Date: 2005-03-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 991-1000 Citation Subset: IM |
Affiliation:
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Comprehensive Cancer Center and Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde-Lyases
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genetics*,
metabolism Chromosome Mapping DNA Primers Escherichia coli / enzymology, genetics* Escherichia coli Proteins / genetics, metabolism Gene Expression Regulation, Bacterial* Gene Expression Regulation, Enzymologic* Genotype Kinetics Plasmids / genetics Promoter Regions, Genetic Substrate Specificity Sugar Acids / metabolism Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Escherichia coli Proteins; 0/Sugar Acids; EC 4.1.2.-/Aldehyde-Lyases; EC 4.1.2.14/phospho-2-keto-3-deoxy-gluconate aldolase |
| Comments/Corrections | |
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