Document Detail


Multiple reaction monitoring for the accurate quantification of amino acids: using hydroxyproline to estimate collagen content.
MedLine Citation:
PMID:  22125153     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Multiple reaction monitoring (MRM) mass spectrometry may be regarded as the gold standard methodology for quantitative mass spectrometry and has been adopted for the analysis of small molecules especially within the pharmaceutical industry. It can also be applied to the analysis of peptides and proteins and to the measurement of the basic building blocks of proteins, amino acids. Here, we describe the application of MRM mass spectrometry to the measurement of hydroxyproline after acid hydrolysis of various animal tissues. We show that the measurement of hydroxyproline provides an accurate and reliable estimate of the collagen content of such tissues and may be a useful indicator of meat tenderness.
Authors:
Michelle L Colgrave; Peter G Allingham; Kerri Tyrrell; Alun Jones
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  828     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  291-303     Citation Subset:  IM    
Affiliation:
CSIRO Division of Livestock Industries, Queensland Bioscience Precinct, St Lucia, QLD, Australia, Michelle.Colgrave@csiro.au.
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