Document Detail


Multiple presentation of scFv800E6 on silica nanospheres enhances binding efficacy toward HER-2 receptor in breast cancer cells.
MedLine Citation:
PMID:  22010849     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Spherical silica nanoparticles (SNP) have been synthesized and functionalized with anti-HER-2 scFv800E6 antibody by both localized histidine-tag recognition, leading to an oriented protein ligation, and glutaraldehyde crosslinking, exploiting a statistical reactivity of lysine amine groups in the primary sequence of the molecule. The targeting efficiency of nanocomplexes in comparison with free scFv was evaluated by flow cytometry using a HER-2 antigen-positive MCF-7 breast cancer cell line, exhibiting a four-fold increase in scFv binding efficacy, close to the affinity of intact anti-HER-2 monoclonal antibody, which suggests the effectiveness of presenting multiple scFv molecules on nanoparticles in improving antigen recognition. Unexpectedly, the conjugation method did not affect the binding efficacy of scFv, suggesting a structural role of lysines in the scFv molecule. Confocal laser scanning microscopy confirmed the binding of nanocomplexes to HER-2 and also provided evidence of their localization at the cell surface.
Authors:
Serena Mazzucchelli; Paolo Verderio; Silvia Sommaruga; Miriam Colombo; Agnese Salvadè; Fabio Corsi; Patrizia Galeffi; Paolo Tortora; Davide Prosperi
Related Documents :
17588219 - Suppression of hepatitis b viral gene expression by protein-tyrosine phosphatase ptpn3.
9390419 - Cloning and characterization of a cdna encoding an elicitor of phytophthora parasitica ...
10650209 - Identification of the cellulose-binding domain of fibrobacter succinogenes endoglucanas...
21385989 - Sedative drug modulates t-cell and lymphocyte function-associated antigen-1 function.
22269039 - B-cell receptor: from resting state to activate.
8931149 - Probing the role of tryptophan residues in a cellulose-binding domain by chemical modif...
7407049 - Ligand interactions with membrane-bound porcine atrial muscarinic receptor(s).
12184999 - Igf-i binding in primary culture of muscle cells of rainbow trout: changes during in vi...
14983039 - Genetic and biophysical studies of diphtheria toxin repressor (dtxr) and the hyperactiv...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-20
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  -     ISSN:  1520-4812     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  How Do We Define Congenital Heart Defects for Scientific Studies?
Next Document:  Inactivation of mitochondrial aspartate aminotransferase contributes to the respiratory deficit of y...