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Multiple-locus variable number of tandem repeats fingerprinting (MLVF) and virulence factor analysis of methicillin resistant Staphylococcus aureus SCCmec type III.
MedLine Citation:
PMID:  22390064     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Methicillin resistant Staphylococcus aureus (MRSA), particularly strains with type III staphylococcal cassette chromosome mec (SCCmec), represent a serious human pathogen in Tehran, Iran. The disease-causing capability depends on their ability to produce a wide variety of virulent factors. The prevalence of exotoxin genes and multiple-locus variable number of tandem repeats fingerprinting (MLVF) profile among MRSA isolates, from patients in Tehran, was evaluated by PCR and Multiplex-PCR. The MLVF typing of 144 MRSA isolates with type III SCCmec produced 5 different MLVF types. Generally, 97.2% (140/144) of all the isolates were positive for at least one of the tested exotoxin genes. The most prevalent genes were hld, found in 87.5% (126/144) of the isolates followed by lukE-lukD and hla found in 72.9% (105/144) and 70.1% (101/144) of the isolates, respectively. The tst gene, belonging to MLVF types I, IV and V, was found among three of the isolates from blood and wound samples. The sea gene was detected in 58.3% (84/144) of the isolates and the sed and see genes were found in one isolate with MLVF type V. The coexistence of genes was observed in the 87.5% (126/144) of the isolates. The rate of coexistence of hld with lukE-lukD, hla with lukE-lukD and sea with lukE-lukD were 66.7% (96/144), 44.4% (64/144) and 44.4% (64/144), respectively. The present study demonstrated that MRSA strains with type III SCCmec show different MLVF patterns and exotoxin profiles.
Authors:
Mohammad Emaneini; Leila Jabalameli; Hossein Iman-Eini; Marzieh Aligholi; Amir Ghasemi; Farrokh Akbari Nakhjavani; Morovat Taherikalani; Babak Khoramian; Parisa Asadollahi; Fereshteh Jabalameli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists     Volume:  60     ISSN:  1733-1331     ISO Abbreviation:  Pol. J. Microbiol.     Publication Date:  2011  
Date Detail:
Created Date:  2012-03-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101229003     Medline TA:  Pol J Microbiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  303-7     Citation Subset:  IM    
Affiliation:
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences. Tehran, Iran.
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