Document Detail

Multiple levels of post-transcriptional regulation of collagenase (matrix metalloproteinase 1) in an epithelial cell line.
MedLine Citation:
PMID:  8436410     Owner:  NLM     Status:  MEDLINE    
Multiple levels of regulation of collagenase (matrix metalloproteinase 1; MMP-1), have been demonstrated in a clonal rat epithelial cell line (A5P/B10). Secreted enzyme could not be demonstrated in culture medium from A5P/B10 cells but, using antibodies specific for collagenase, the enzyme was detected within the cytoplasm and on the surface of the cells. A probe for rat collagenase could not detect a signal for mRNA in the cytoplasm while nuclear run-on data demonstrated that the gene for collagenase was being transcribed. Incubating the cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) significantly increased cytoplasmic mRNA levels and slightly increased the intensity of staining in permeabilized cells, but collagenase activity was still not detected in the conditioned medium. This indicated that the protein was being synthesized by the TPA-treated cells but was not being secreted into the medium. These data suggest that the secretion of collagenase may be regulated both following transcription and after the completion of translation and it is suggested that multiple levels of control may be operating to determine the rate of collagenase release and hence, the rate of collagen turnover.
J M Whitelock; M L Paine; J R Gibbins; R F Kefford; R L O'Grady
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology and cell biology     Volume:  71 ( Pt 1)     ISSN:  0818-9641     ISO Abbreviation:  Immunol. Cell Biol.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-03-23     Completed Date:  1993-03-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8706300     Medline TA:  Immunol Cell Biol     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  39-47     Citation Subset:  IM    
Department of Pathology and Immunology, University of Technology, Gore Hill, Australia.
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MeSH Terms
Cell Line
Cell Membrane / metabolism
Cells, Cultured
Chromosome Deletion
Collagen / metabolism
Collagenases / genetics*,  metabolism*
Culture Media
Cytoplasm / metabolism
DNA Probes
Epithelium / metabolism
Gene Expression Regulation, Enzymologic*
Gene Rearrangement
Matrix Metalloproteinase 1
Protein Processing, Post-Translational*
RNA, Messenger / metabolism
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Culture Media; 0/DNA Probes; 0/RNA, Messenger; 16561-29-8/Tetradecanoylphorbol Acetate; 9007-34-5/Collagen; EC 3.4.24.-/Collagenases; EC Metalloproteinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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