| Multiple injections of coloured microspheres for islet blood flow measurements in anaesthetised rats: influence of microsphere size. | |
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MedLine Citation:
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PMID: 12602783 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated if coloured microspheres could be used for repeated measurements of pancreatic islet blood flow in rats. An initial injection of 1.0-1.5 x 10(5) microspheres (black colour), with a size of 10 or 15 microm, was made into the ascending aorta, while an arterial reference sample was collected from the femoral artery. Twelve min later, 1 ml of saline or 30% D-glucose was injected intravenously. Three min after this injection a second injection of 10- or 15-microm microspheres (green colour) was given. The animals were then killed, and the pancreas and adrenals were removed and samples (150-200 mg) were secured from the duodenum, ileum, colon, right kidney and liver. The microsphere contents were determined with the aid of a freeze-thawing technique and blood flow values were calculated. Our results suggest that 10-microm microspheres, but not 15-microm microspheres, provide reproducible islet and total pancreatic blood flow measurements when repeatedly injected. Values for the blood flow to the intestines, kidney and liver were less sensitive to the size of the microspheres. We conclude that repeated administration of 15-microm microspheres induces a high risk for erroneous islet and total pancreatic blood flow measurements, whereas two such measurements can be performed if 10-microm microspheres are used. |
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Authors:
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Per-Ola Carlsson; Orjan Källskog; Birgitta Bodin; Arne Andersson; Leif Jansson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Upsala journal of medical sciences Volume: 107 ISSN: 0300-9734 ISO Abbreviation: Ups. J. Med. Sci. Publication Date: 2002 |
Date Detail:
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Created Date: 2003-02-26 Completed Date: 2003-06-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0332203 Medline TA: Ups J Med Sci Country: Sweden |
Other Details:
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Languages: eng Pagination: 111-20 Citation Subset: IM |
Affiliation:
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Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden. Per-Ola.Carlsson@medcellbiol.uu.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta Color Freezing Injections, Intra-Arterial Intestines / blood supply Islets of Langerhans / blood supply* Liver Circulation Male Microspheres* Pancreas / blood supply Particle Size Rats Rats, Sprague-Dawley Regional Blood Flow Renal Circulation Reproducibility of Results Sensitivity and Specificity |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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