Document Detail


Multiple initial culture conditions enhance the establishment of cell lines from primary ovarian cancer specimens.
MedLine Citation:
PMID:  16759149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To increase the efficiency of stable cell line establishment from primary ovarian cancer specimens, we simultaneously initiated cultures under multiple conditions, varying extracellular matrices and the inclusion of supplements (e.g., serum or serum albumin), while minimizing exposure to xenogeneic antigens (e.g., fetal calf serum). Primary cultures were initiated from 30 specimens; cell lines were established from 10 of these for a success rate of 33%. In some instances, multiple cell lines were established from the same specimen. Five lines were characterized extensively with respect to growth properties, antigen expression, and genomic alterations. Although these lines are all low-passage, marked heterogeneity was observed, even between lines derived from the same specimen. The culture approach outlined herein will facilitate generation of reagents useful for many aspects of ovarian cancer biology.
Authors:
Cara C Bertozzi; Cheng-Yi Chang; Sonya Jairaj; Xiaochuan Shan; Jia Huang; Barbara L Weber; Christina S Chu; Richard G Carroll
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  42     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2006 Mar-Apr
Date Detail:
Created Date:  2006-06-08     Completed Date:  2006-12-27     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  United States    
Other Details:
Languages:  eng     Pagination:  58-62     Citation Subset:  IM    
Affiliation:
Abramson Family Cancer Research Institute, Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19104-6160, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques*
Cell Line*
Cells, Cultured
Female
Humans
Ovarian Neoplasms / pathology*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA-113783/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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