Document Detail

Multiple inert gas elimination technique for determining ventilation/perfusion distributions in rat during normoxia, hypoxia and hyperoxia.
MedLine Citation:
PMID:  11380516     Owner:  NLM     Status:  MEDLINE    
1. The use of the multiple inert gas elimination technique (MIGET) in quantifying ventilation/perfusion distributions (V*A/Q*) in small animals, such as the rat, may cause results to be biased due to haemodilution produced by the large volume of liquid infused intravenously. 2. We tested two methods of administering inert gases in rats using the MIGET: (i) standard continuous intravenous administration of inert gases (method A); and (ii) a new method based on the physicochemical properties of each inert gas (method B). This method included acute simultaneous inert gas administration using three pathways: inhalation, intravenous infusion and rectal infusion. Both MIGET methods were applied to obtain data while breathing three different inspiratory fractions of oxygen (FIO2): normoxia, hypoxia and hyperoxia. 3. Inert gas levels obtained from blood or expired air samples were sufficient for chromatographic measurement, at least during a 2 h period. The V*A/Q* distributions reported using both methods were acceptable for all the physiological conditions studied; therefore, the alternative method used here may be useful in further MIGET studies in rats because haemodilution resulting from continuous intravenous infusion of less-soluble gases can be avoided. 4. Normoxic rats showed lower mean values of the V*A/Q* ratio of ventilation distribution and higher mean values of the V*A/Q* ratio of perfusion distribution with the usual method of inert gas administration (method A). These non-significant differences were observed under almost all physiological conditions studied and they could be caused by haemodilution. Nevertheless, the effect of interindividual differences cannot be discarded. An additional effect of the low haematocrit on cardiovascular changes due to low FIO2, such as pulmonary vasoconstriction or increased cardiac output, may explain the lower dispersion of perfusion distributions found in group A during hypoxia.
V Alfaro; J Roca-Acín; L Palacios; R Guitart
Related Documents :
22358906 - Separation of growth-stimulating activity of bsa fraction v from the bulk of albumin us...
22752446 - Determination of terbuthylazine and desethylterbuthylazine in human urine and hair samp...
21776476 - Application of response surface methodology to optimize pressurized liquid extraction o...
22435356 - Ionic liquids: differential scanning calorimetry as a new indirect method for determina...
22944006 - On-line sample concentration and determination of cationic alkaloids in human plasma by...
16256706 - Evaluation of lc methods for the separation of phenoxymethylpenicillin and its related ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  28     ISSN:  0305-1870     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:    2001 May-Jun
Date Detail:
Created Date:  2001-05-30     Completed Date:  2001-07-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  419-24     Citation Subset:  IM    
Department of Physiology, University of Barcelona, Barcelona, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anoxia / physiopathology*
Gases / diagnostic use*
Hyperoxia / physiopathology*
Oxygen Consumption / drug effects,  physiology
Rats, Sprague-Dawley
Ventilation-Perfusion Ratio / physiology*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effect of anti-oxidant treatment and cholesterol lowering on resting arterial tone, metabolic vasodi...
Next Document:  Cyclo-oxygenase 2 tissue distribution and developmental pattern of expression in the chicken.