Document Detail


Multiple in vivo passages enhance the ability of a clinical Helicobacter pylori isolate to colonize the stomach of Mongolian gerbils and to induce gastritis.
MedLine Citation:
PMID:  15901366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Mongolian gerbil is an excellent animal model for Helicobacter pylori-induced gastritis in humans. In this study, initially low colonization rates of the H. pylori strains ATCC 43504, SS1, or HP87 inoculated into gerbils caused difficulties in establishing this model. In order to increase the colonization ability and pathogenicity, the clinical HP87 isolate was selected for adaptation to the gerbil stomach by multiple in vivo passages through gerbils. Development of gastritis was examined histologically at 4-52 weeks after infection. The proportion of gerbils which tested positive for H. pylori by culture at four weeks after inoculation gradually increased from 11.1% of gerbils inoculated with HP87 without prior in vivo passage (P0) to 100% of gerbils inoculated with HP87 with seven in vivo passages (P7). In addition, adaptation of HP87 resulted in more severe histopathological changes. Gerbils infected with adapted HP87 (P7) exhibited severe infiltration by monomorphonuclear and polymorphonuclear leukocytes in the mucosa, submucosa, and subserosa of the gastric antrum, as well as epithelial changes consisting of hyperplasia, erosion, and ulceration. Histopathological changes increased in severity from four to 52 weeks after infection. Adaptation of HP87 during its passages through gerbils could be due to genetic changes in bacterial colonization factors. Identification of these changes might be useful to understand the underlying mechanism of gastric adaptation and pathogenesis of H. pylori.
Authors:
A Bleich; I Köhn; S Glage; W Beil; S Wagner; M Mähler
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Laboratory animals     Volume:  39     ISSN:  0023-6772     ISO Abbreviation:  Lab. Anim.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-05-19     Completed Date:  2005-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0112725     Medline TA:  Lab Anim     Country:  England    
Other Details:
Languages:  eng     Pagination:  221-9     Citation Subset:  IM    
Affiliation:
Institute for Laboratory Animal Science and Central Animal Facility, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. bleich.andre@mh-hannover.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Colony Count, Microbial
Gastritis / microbiology*
Gerbillinae
Helicobacter Infections / microbiology*,  pathology
Helicobacter pylori / pathogenicity*
Histological Techniques
Pyloric Antrum / pathology
Serial Passage / methods*
Species Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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