| Multiple in vivo liver biopsies using a freeze-clamping technique. | |
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MedLine Citation:
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PMID: 12028621 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A multiple in vivo liver biopsy technique was developed to measure labile metabolites (creative phosphate [CP], ATP, lactate) without interfering with normal perfusion and metabolism. Anesthetized rats (n = 7) had a midline abdominal incision done to expose the liver. Four biopsies were taken across 20 min. Bleeding was controlled by a small, nontraumatic clamp proximal to the biopsy. Prefrozen dressing forceps grasped the liver and scissors cut the biopsy, which was frozen in liquid nitrogen. Bleeding was minimal and hemodynamic stability was preserved. This technique has few complications, bleeding is easily controlled, and it allows for large multiple biopsies, which give reliable metabolic data and can be consistently taught. |
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Authors:
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Michael P Belanger; Nesime Askin; Carin Wittnich |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of investigative surgery : the official journal of the Academy of Surgical Research Volume: 15 ISSN: 0894-1939 ISO Abbreviation: J Invest Surg Publication Date: 2002 Mar-Apr |
Date Detail:
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Created Date: 2002-05-24 Completed Date: 2002-10-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8809255 Medline TA: J Invest Surg Country: United States |
Other Details:
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Languages: eng Pagination: 109-12 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Toronto, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biopsy / instrumentation, methods* Freezing* Hemorrhage / prevention & control Liver / pathology* Rats Rats, Sprague-Dawley Surgical Instruments |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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