Document Detail

Multiple immunoreactive forms of met- and leu-enkephalin in fetal and neonatal rat brain and in rat gut.
MedLine Citation:
PMID:  7154839     Owner:  NLM     Status:  MEDLINE    
We have used radioimmunoassays with carboxyl-specific antisera to study the development of met- and leu-enkephalin in rat brain and gut from 13-days of fetal age through adulthood. Fractionation of HCl extracts of fetal and neonatal brain tissues by HPLC revealed the presence of immunoreactive forms other than met- and leu-enkephalin. For example, HPLC separation of extracts of 16-day fetal brain yielded two peaks of leu-enkephalin-like immunoreactivity. One emerged at the position of leu-enkephalin, the other eluted with about one-third the retention time. There were four peaks of met-enkephalin-like immunoreactivity, one with the retention time characteristic of met-enkephalin, the others with shorter retention times. In contrast, all of the met-enkephalin-like immunoreactivity in adult brain eluted with the retention time characteristic of authentic met-enkephalin and all of the leu-enkephalin-like immunoreactivity eluted in the position of authentic leu-enkephalin. Multiple immunoreactive forms of met- and leu-enkephalin were found in extracts of both fetal and adult gut tissues.
J L Dahl; M L Epstein; B L Silva; I Lindberg
Related Documents :
15810879 - Englitazone administration to late pregnant rats produces delayed body growth and insul...
11464959 - Fetal and neonatal neurologic consultations: identifying brain disorders in the context...
18236399 - Safe--the special non-invasive advances in fetal and neonatal evaluation network: aims ...
3900839 - Effects of ultrasound feedback on pregnancy anxiety, fetal activity, and neonatal outcome.
20584799 - The management of heroin misuse in pregnancy: time for a rethink?
6469839 - Prevalence of oocysts of isospora suis and eimeria spp from sows on farms with and with...
2850989 - The human placental bed: histology, immunohistochemistry and pathology.
18986549 - Lack of functional alpha-lactalbumin prevents involution in cape fur seals and identifi...
10428519 - Administration of low-dose aspirin to mothers with small for gestational age fetuses an...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Life sciences     Volume:  31     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:    1982 Oct 18-25
Date Detail:
Created Date:  1983-03-24     Completed Date:  1983-03-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1853-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Newborn / immunology*
Brain / immunology*
Chromatography, High Pressure Liquid
Enkephalin, Leucine / immunology*
Enkephalin, Methionine / immunology*
Fetus / immunology*
Intestines / immunology*
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
58569-55-4/Enkephalin, Methionine; 58822-25-6/Enkephalin, Leucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Metabolism of a heptapeptide opioid by rat brain and cardiac tissue.
Next Document:  Peptide F (pro-enkephalin fragment): radioimmunoassay, and stress-induced changes in adrenal.