Document Detail


Multiple immunoreactive forms of met- and leu-enkephalin in fetal and neonatal rat brain and in rat gut.
MedLine Citation:
PMID:  7154839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have used radioimmunoassays with carboxyl-specific antisera to study the development of met- and leu-enkephalin in rat brain and gut from 13-days of fetal age through adulthood. Fractionation of HCl extracts of fetal and neonatal brain tissues by HPLC revealed the presence of immunoreactive forms other than met- and leu-enkephalin. For example, HPLC separation of extracts of 16-day fetal brain yielded two peaks of leu-enkephalin-like immunoreactivity. One emerged at the position of leu-enkephalin, the other eluted with about one-third the retention time. There were four peaks of met-enkephalin-like immunoreactivity, one with the retention time characteristic of met-enkephalin, the others with shorter retention times. In contrast, all of the met-enkephalin-like immunoreactivity in adult brain eluted with the retention time characteristic of authentic met-enkephalin and all of the leu-enkephalin-like immunoreactivity eluted in the position of authentic leu-enkephalin. Multiple immunoreactive forms of met- and leu-enkephalin were found in extracts of both fetal and adult gut tissues.
Authors:
J L Dahl; M L Epstein; B L Silva; I Lindberg
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Life sciences     Volume:  31     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:    1982 Oct 18-25
Date Detail:
Created Date:  1983-03-24     Completed Date:  1983-03-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1853-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / immunology*
Brain / immunology*
Chromatography, High Pressure Liquid
Enkephalin, Leucine / immunology*
Enkephalin, Methionine / immunology*
Female
Fetus / immunology*
Intestines / immunology*
Male
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
NS 16280/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
58569-55-4/Enkephalin, Methionine; 58822-25-6/Enkephalin, Leucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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