| Multiple forms of tubulin in the cytoskeletal and flagellar microtubules of Polytomella. | |
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MedLine Citation:
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PMID: 7309786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The alga polytomella contains several organelles composed of microtubules, including four flagella and hundreds of cytoskeletal microtubules. Brown and co-workers have shown (1976. J. Cell Biol. 69:6-125; 1978, Exp. Cell Res. 117: 313-324) that the flagella could be removed and the cytoskeletans dissociated, and that both structures could partially regenerate in the absence of protein synthesis. Because of this, and because both the flagella and the cytoskeletons can be isolated intact, this organism is particularly suitable for studying tubulin heterogeneity and the incorporation of specific tubulins into different microtubule-containing organelles in the same cell. In order to define the different species of tubulin in polytonella cytoplasm, a (35)S- labeled cytoplasmic fraction was subjected to two cycles of assembly and disassembly in the presence of unlabeled brain tubulin. Comparison of the labeled polytomella cytoplasmic tubulin obtained by this procedure with the tubulin of isolated polytomella flagella by two-dimensional gel electrophoresis showed that, whereas the beta-tubulin from both cytoplasmic and flagellar tubulin samples comigrated, the two alpha-tubulins had distinctly different isoelectic points. As a second method of isolating tubulin from the cytoplasm, cells were gently lysed with detergent and intact cytoskeletons obtained. When these cytoskeletons were exposed to cold temperature, the proteins that were released were found to be highly enriched in tubulin; this tubulin, by itself, could be assembled into microtubules in vitro. The predominant alpha-tubulin of this in vitro- assembled cytoskeletal tubulin corresponded to the major cytoplasmic alpha-tubulin obtained by coassembly of labeled polytomella cytoplasmic extract with brain tubulin and was quite distinct from the alpha-tubulin of purified flagella. These results clearly show that two different microtubule-containing organelles from the same cell are composed of distinct tubulins. |
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Authors:
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T W McKeithan; J L Rosenbaum |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of cell biology Volume: 91 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1981 Nov |
Date Detail:
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Created Date: 1982-02-12 Completed Date: 1982-02-12 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 352-60 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Algae
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analysis*,
ultrastructure Cytoplasm / analysis Electrophoresis, Polyacrylamide Gel Flagella / ultrastructure Isoelectric Point Microtubules / analysis* Polymers Tubulin / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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GM 14,642/GM/NIGMS NIH HHS; GM02044/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Polymers; 0/Tubulin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol Journal ID (publisher-id): jcb ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 11 Year: 1981 Volume: 91 Issue: 2 First Page: 352 Last Page: 360 ID: 2111981 Publisher Id: 912352 PubMed Id: 7309786 |
| Multiple forms of tubulin in the cytoskeletal and flagellar microtubules of polytomella | |
| TW McKeithan | |
| JL Rosenbaum | |
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