Document Detail


Multiple forms of acid phosphatase and their differential secretion during stalk formation under submerged monolayer incubation of Dictyostelium discoideum.
MedLine Citation:
PMID:  3779849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The electrophoretic pattern of intracellular and secreted acid phosphatases (AcPases) in Dictyostelium discoideum was examined during incubation of the cells as a submerged monolayer. Three distinct forms of the enzyme were observed in the cell during differentiation; one was detected throughout development (AcPase 1), whereas the others including AcPase 2 were stage-specific. AcPase 1 was released in the medium predominantly in early development and AcPase 2, a prestalk specific form, was secreted during stalk formation. When cells were incubated under conditions where stalk cells did not form, only AcPase 1 was recognized both in the cell and in the medium.
Authors:
A A Oohata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell differentiation     Volume:  19     ISSN:  0045-6039     ISO Abbreviation:  Cell Differ.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1986-12-24     Completed Date:  1986-12-24     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0342640     Medline TA:  Cell Differ     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  271-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / secretion*
Cell Differentiation
Dictyostelium / enzymology*
Electrophoresis, Polyacrylamide Gel
Isoenzymes / secretion*
Lysosomes / enzymology
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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