Document Detail


Multiple endoxylanases of Butyrivibrio sp.
MedLine Citation:
PMID:  11501489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Butyrivibrio sp. Mz 5 with a high xylanolytic activity was isolated. Four major xylanases were detected in the cell-associated fraction using the zymogram technique. The xylanolytic activity was inducible with the oat spelts xylan; two endoxylanases (51 and 145 kDa) were formed constitutively. The bulk of the xylanolytic activity was cell-bound and growth-phase dependent; the maximum activity in the cell-associated fraction was achieved after 16 h of incubation. The highest xylanolytic activity was determined in a medium with 0.5% oat spelts xylan. Under optimum conditions (the highest xylanolytic activity produced), the two cell-bound xylanases (51 and 58 kDa) were isolated by anion exchange chromatography on CIM DEAE 8 tubes attached to a MPLC system, and gel filtration.
Authors:
M Zorec; T Cepeljnik; F V Nekrep; R M Logar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia microbiologica     Volume:  46     ISSN:  0015-5632     ISO Abbreviation:  Folia Microbiol. (Praha)     Publication Date:  2001  
Date Detail:
Created Date:  2001-08-14     Completed Date:  2001-10-11     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376757     Medline TA:  Folia Microbiol (Praha)     Country:  Czech Republic    
Other Details:
Languages:  eng     Pagination:  94-6     Citation Subset:  IM    
Affiliation:
Zootechnical Department, Biotechnical Faculty, University of Ljubljana, 1230 Domzale, Slovenia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Culture Media
Electrophoresis, Polyacrylamide Gel
Endo-1,4-beta Xylanases
Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology*,  growth & development
Rumen / microbiology
Xylosidases / chemistry,  isolation & purification,  metabolism*
Chemical
Reg. No./Substance:
0/Culture Media; EC 3.2.1.-/Xylosidases; EC 3.2.1.8/Endo-1,4-beta Xylanases

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