Document Detail

Multiple-Particle-Tracking to investigate viscoelastic properties in living cells.
MedLine Citation:
PMID:  20035872     Owner:  NLM     Status:  MEDLINE    
Cell mechanical properties play an important role in determining many cellular activities. Passive microrheology techniques, such as Multiple-Particle-Tracking (MPT) give an insight into the structural rearrangements and viscoelastic response of a wide range of materials, in particular soft materials and complex fluids like cell cytoplasm in living cells. The technique finds an important field of application in large cells such as oocytes where, during their growth, several organelles and molecules are displaced in specific territories of the cell instrumental for later embryonic development. To measure cell mechanics, cells are usually deformed by many techniques that are slow and often invasive. To overcome these limits, the MPT technique is applied. Probe particles are embedded in the viscoelastic sample and their properties are extracted from the thermal fluctuation spectra measured using digital video-microscopy. The Brownian motion of a probe particle immersed in a network is directly related to the network's mechanical properties. Particles exhibit larger motions when their local environments are less rigid or less viscous. The mean-square-displacement (MSD) of the particle's trajectory is used to quantify its amplitude of motions over different time scales.
Lara Selvaggi; Marinella Salemme; Carmen Vaccaro; Giuseppe Pesce; Giulia Rusciano; Antonio Sasso; Chiara Campanella; Rosa Carotenuto
Publication Detail:
Type:  Journal Article; Review     Date:  2009-12-24
Journal Detail:
Title:  Methods (San Diego, Calif.)     Volume:  51     ISSN:  1095-9130     ISO Abbreviation:  Methods     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-11     Completed Date:  2010-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426302     Medline TA:  Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  20-6     Citation Subset:  IM    
Physics Department, University of Naples Federico II, via Cinthia, Napoli, Italy.
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MeSH Terms
Biophysics / methods*
COS Cells
Cercopithecus aethiops
Cytological Techniques
Cytoplasm / metabolism
Microscopy, Video / methods
Models, Biological
Oligonucleotide Probes / metabolism
Oocytes / metabolism
Particle Size
Xenopus laevis / metabolism
Reg. No./Substance:
0/Oligonucleotide Probes

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