Document Detail

Multiple factors influence glomerular albumin permeability in rats.
MedLine Citation:
PMID:  22223875     Owner:  NLM     Status:  MEDLINE    
Different laboratories recently reported incongruous results describing the quantification of albumin filtration using two-photon microscopy. We investigated the factors that influence the glomerular sieving coefficient for albumin (GSC(A)) in an effort to explain these discordant reports and to develop standard operating procedures for determining GSC(A). Multiple factors influenced GSC(A), including the kidney depth of image acquisition (10-20 μm was appropriate), the selection of fluorophore (probes emitting longer wavelengths were superior), the selection of plasma regions for fluorescence measurements, the size and molecular dispersion characteristics of dextran polymers if used, dietary status, and the genetic strain of rat. Fasting reduced the GSC(A) in Simonsen Munich Wistar rats from 0.035±0.005 to 0.016±0.004 (P<0.01). Frömter Munich Wistar rats had a much lower GSC(A) in both the fed and the fasted states. Finally, we documented extensive albumin transcytosis with vesicular and tubular delivery to and fusion with the basolateral membrane in S1 proximal tubule cells. In summary, these results help explain the previously conflicting microscopy and micropuncture data describing albumin filtration and highlight the dynamic nature of glomerular albumin permeability.
Ruben M Sandoval; Mark C Wagner; Monica Patel; Silvia B Campos-Bilderback; George J Rhodes; Exing Wang; Sarah E Wean; Sherry S Clendenon; Bruce A Molitoris
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-01-05
Journal Detail:
Title:  Journal of the American Society of Nephrology : JASN     Volume:  23     ISSN:  1533-3450     ISO Abbreviation:  J. Am. Soc. Nephrol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-01     Completed Date:  2012-04-24     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9013836     Medline TA:  J Am Soc Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  447-57     Citation Subset:  IM    
Division of Nephrology, Department of Medicine, Indiana Center for Biological Microscopy, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
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MeSH Terms
Albumins / metabolism*
Cell Membrane Permeability / physiology*
Fluorescent Dyes
Kidney Glomerulus / cytology*,  physiology*
Kidney Tubules, Proximal / cytology*,  physiology*
Microscopy, Fluorescence, Multiphoton
Models, Animal
Rats, Sprague-Dawley
Rats, Wistar
Time Factors
Grant Support
Reg. No./Substance:
0/Albumins; 0/Fluorescent Dyes; 9004-54-0/Dextrans
Comment In:
J Am Soc Nephrol. 2012 Mar;23(3):373-5   [PMID:  22323639 ]

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