| Multiple approaches to analyse the data for rat brain acetylcholinesterase inhibition by cyclophosphamide. | |
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MedLine Citation:
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PMID: 20652634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acetylcholinesterase (AChE) is an externally oriented membrane-bound enzyme and its main physiological role is termination of chemical transmission at cholinergic synapses and secretory organs by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). Nevertheless, it is well known that cyclophosphamide (CP; nitrogen mustard derivative) is an eminent anticancer drug. The present work addresses multiple approaches to analyze an identical data for rat brain AChE inhibition by CP. These different angels of analysis based on two classical (Lineweaver-Burk as well as Dixon) plots, their secondary replots, a new graphical approach and general built-in equations of GOSA. Thus various kinetic constants (K(I), K(s,) K(m), k(sl), V(mao), K(i), k(sli), S(lo), K(maxi), S(K0.5), k(cat) and k(sp)) were estimated and mode of inhibition discussed in the current study. |
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Authors:
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Mohammad A Kamal; M Reale; Abdulaziz A Al-Jafari |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-21 |
Journal Detail:
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Title: Neurochemical research Volume: 35 ISSN: 1573-6903 ISO Abbreviation: Neurochem. Res. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-20 Completed Date: 2011-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: United States |
Other Details:
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Languages: eng Pagination: 1501-9 Citation Subset: IM |
Affiliation:
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Enzymoic, 7 Peterlee Pl., Hebersham, NSW, 2770, Australia. ma.kamal@live.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholinesterase
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metabolism* Animals Brain / drug effects*, enzymology Cholinesterase Inhibitors / pharmacology* Cyclophosphamide / pharmacology* Kinetics Male Mathematical Concepts Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Cholinesterase Inhibitors; 50-18-0/Cyclophosphamide; EC 3.1.1.7/Acetylcholinesterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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