Document Detail


Multiphysics simulation of a microfluidic perfusion chamber for brain slice physiology.
MedLine Citation:
PMID:  20464499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Understanding and optimizing fluid flows through in vitro microfluidic perfusion systems is essential in mimicking in vivo conditions for biological research. In a previous study a microfluidic brain slice device (microBSD) was developed for microscale electrophysiology investigations. The device consisted of a standard perfusion chamber bonded to a polydimethylsiloxane (PDMS) microchannel substrate. Our objective in this study is to characterize the flows through the microBSD by using multiphysics simulations of injections into a pourous matrix to identify optimal spacing of ports. Three-dimensional computational fluid dynamic (CFD) simulations are performed with CFD-ACE + software to model, simulate, and assess the transport of soluble factors through the perfusion bath, the microchannels, and a material that mimics the porosity, permeability and tortuosity of brain tissue. Additionally, experimental soluble factor transport through a brain slice is predicted by and compared to simulated fluid flow in a volume that represents a porous matrix material. The computational results are validated with fluorescent dye experiments.
Authors:
Hector H Caicedo; Maximiliano Hernandez; Christopher P Fall; David T Eddington
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical microdevices     Volume:  12     ISSN:  1572-8781     ISO Abbreviation:  Biomed Microdevices     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-08-05     Completed Date:  2011-01-10     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  United States    
Other Details:
Languages:  eng     Pagination:  761-7     Citation Subset:  IM    
Affiliation:
Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / cytology,  physiology*
Dimethylpolysiloxanes / chemistry
Mice
Microfluidic Analytical Techniques*
Microscopy, Fluorescence
Models, Biological*
Perfusion / instrumentation*
Physics*
Porosity
Grant Support
ID/Acronym/Agency:
MH-085073/MH/NIMH NIH HHS; MH-64611/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Dimethylpolysiloxanes; 63148-62-9/baysilon
Comments/Corrections

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