| Multiphoton spectral analysis of benzo[a]pyrene uptake and metabolism in a rat liver cell line. | |
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MedLine Citation:
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PMID: 21420996 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dynamic analysis of the uptake and metabolism of polycyclic aromatic hydrocarbons (PAHs) and their metabolites within live cells in real time has the potential to provide novel insights into genotoxic and non-genotoxic mechanisms of cellular injury caused by PAHs. The present work, combining the use of metabolite spectra generated from metabolite standards using multiphoton spectral analysis and an "advanced unmixing process", identifies and quantifies the uptake, partitioning, and metabolite formation of one of the most important PAHs (benzo[a]pyrene, BaP) in viable cultured rat liver cells over a period of 24 h. The application of the advanced unmixing process resulted in the simultaneous identification of 8 metabolites in live cells at any single time. The accuracy of this unmixing process was verified using specific microsomal epoxide hydrolase inhibitors, glucuronidation and sulfation inhibitors as well as several mixtures of metabolite standards. Our findings prove that the two-photon microscopy imaging surpasses the conventional fluorescence imaging techniques and the unmixing process is a mathematical technique that seems applicable to the analysis of BaP metabolites in living cells especially for analysis of changes of the ultimate carcinogen benzo[a]pyrene-r-7,t-8-dihydrodiol-t-9,10-epoxide. Therefore, the combination of the two-photon acquisition with the unmixing process should provide important insights into the cellular and molecular mechanisms by which BaP and other PAHs alter cellular homeostasis. |
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Authors:
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Rola Barhoumi; Youssef Mouneimne; Ernesto Ramos; Christophe Morisseau; Bruce D Hammock; Stephen Safe; Alan R Parrish; Robert C Burghardt |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural Date: 2011-03-21 |
Journal Detail:
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Title: Toxicology and applied pharmacology Volume: 253 ISSN: 1096-0333 ISO Abbreviation: Toxicol. Appl. Pharmacol. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-25 Completed Date: 2011-06-20 Revised Date: 2012-05-18 |
Medline Journal Info:
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Nlm Unique ID: 0416575 Medline TA: Toxicol Appl Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 45-56 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843-4458, USA. rmouneimne@cvm.tamu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzo(a)pyrene / metabolism* Biological Transport / physiology Cell Line Liver / cytology*, metabolism* Microscopy, Fluorescence, Multiphoton* / methods Rats |
| Grant Support | |
ID/Acronym/Agency:
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1S10RR22532-01/RR/NCRR NIH HHS; N02-CB-07008/CB/NCI NIH HHS; P30 ES009106-09/ES/NIEHS NIH HHS; P30-ES09106/ES/NIEHS NIH HHS; P42 ES004917-11/ES/NIEHS NIH HHS; P42-ES04917/ES/NIEHS NIH HHS; R01 ES002710/ES/NIEHS NIH HHS; R01 ES002710-30/ES/NIEHS NIH HHS; R01 ES002710-31/ES/NIEHS NIH HHS; T32 ES007273-07/ES/NIEHS NIH HHS; T32 ES07273/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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50-32-8/Benzo(a)pyrene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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