Document Detail

Multiparameter morphology as a means of specifically identifying hormone stimulated adrenal cortical tumor cells (Y-1) and granulosa cells in coculture.
MedLine Citation:
PMID:  6330879     Owner:  NLM     Status:  MEDLINE    
In order to test for biologically valid, direct communication between mouse adrenal tumor and porcine ovarian granulosa cells a method was needed for distinguishing the two cell types in culture. To this end granulosa cells were cultured for 24-48 hours after which they were challenged with rhodamine conjugated latex beads for 30 minutes to 24 hours in monolayer culture. When phagocytosis was allowed to occur for longer than one hour the phagocytic vesicles containing the conjugated beads appeared to fuse with each other and with digestive vacuoles. The resultant vesicles contained many beads and were located within the cell body close to the nucleus. At shorter incubation periods, beads were observed predominantly in the periphery of the cell. Y-1 adrenal cortical tumor cells failed to ingest beads and could be distinguished from the bead labeled granulosa cell in coculture with scanning or thin section electron microscopic as well as fluorescence microscopic techniques. Gap junctions were observed between heterotypic cell pairs; however, no evidence of cell fusion was found. ACTH stimulated granulosa: Y-1 adrenal cocultures initiated cAMP-dependent kinase dissociation in the Y-1 cells and in contacting granulosa cells identified positively by the presence of the beads. We have used the bead labeling technique and a procedure for following the kinetics of cAMP-dependent protein kinase dissociation in order to follow the specific hormonal stimulation of these heterotypic cells in coculture.(ABSTRACT TRUNCATED AT 250 WORDS)
S A Murray; W H Fletcher
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Scanning electron microscopy     Volume:  -     ISSN:  0586-5581     ISO Abbreviation:  Scan Electron Microsc     Publication Date:  1984  
Date Detail:
Created Date:  1984-07-30     Completed Date:  1984-07-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0371617     Medline TA:  Scan Electron Microsc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  401-7     Citation Subset:  IM    
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MeSH Terms
Adrenal Cortex Neoplasms / physiopathology*,  ultrastructure
Adrenocorticotropic Hormone / pharmacology*
Cell Communication* / drug effects*
Cell Line
Cells, Cultured
Granulosa Cells / physiology*,  ultrastructure
Microscopy, Electron / methods
Microscopy, Electron, Scanning / methods
Microscopy, Fluorescence / methods
Phagocytosis / drug effects
Protein Kinases / metabolism
Grant Support
Reg. No./Substance:
9002-60-2/Adrenocorticotropic Hormone; EC 2.7.-/Protein Kinases

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