Document Detail


Multinucleate host cells induced by Vittaforma corneae (Microsporidia).
MedLine Citation:
PMID:  16004369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The microsporidium Vittaforma corneae develops within the target cell cytoplasm. In the present study, green monkey kidney (E6) cells infected at 30 degrees C, 35 degrees C or 37 degrees C with V. corneae developed enlarged multinucleate structures of up to 200 microm in any horizontal dimension made up either of a single cell or of multiple fused cells. A number of epithelial cell types (SW-480, HT-29, Caco-2 and HCT-8) were infected with V. corneae but did not induce the same highly organized structures, suggesting that for the structure to develop, the host cell must be capable of continued mitosis, and not be differentiated or be detaching from the surface matrix. Live cell imaging of infected E6 cells revealed large, multinucleate infected cells characterized by a central focus from which radiated parasite stages and host cell mitochondria. Immunocytochemistry identifying gamma and alpha tubulin suggested that a single centrally-located microtubule organizing centre governed the distribution of parasite stages and host cell organelles, with mitochondria and parasites being eventually transported towards the periphery of the structure. Whole cell patch clamp analysis of infected cells indicated an average five-fold increase in total membrane capacitance, consistent with an enlarged single cell. Scanning electron microscopy revealed cell-like protrusions around the periphery of the structure with the intervening space being made up of parasites and cell debris. Clearly in the case of V. corneae-infected E6 cells the parasite-host cell relationship involves subverting the host cell cytoskeleton and cell volume control, providing the parasite with the same protected niche as does a xenoma.
Authors:
Gordon J Leitch; Andrew P Shaw; Margaret Colden-Stanfield; Mary Scanlon; Govinda S Visvesvara
Related Documents :
12181449 - Role of the human herpesvirus 6 u69-encoded kinase in the phosphorylation of ganciclovir.
1604819 - A 44,000 glycoprotein is involved in the attachment of echovirus-11 onto susceptible ce...
12556989 - Effect of ca2+-dependent cell death on the release of herpes simplex virus.
19504579 - A flow cytometric assay for the study of e3 ubiquitin ligase activity.
4112069 - A bacteriophage of bacillus subtilis which forms plaques only at temperatures above 50 ...
14738979 - Development of an in situ hybridisation procedure for the detection of sole aquabirnavi...
21410509 - Flagella and pili are both necessary for efficient attachment of methanococcus maripalu...
6693509 - Hexose transport derepressed and refractory to purine regulation in nad(h)-depleted nil...
12173659 - Chemical responses of single yeast cells studied by fluorescence microspectroscopy unde...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Folia parasitologica     Volume:  52     ISSN:  0015-5683     ISO Abbreviation:  Folia Parasitol.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-07-11     Completed Date:  2005-08-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0065750     Medline TA:  Folia Parasitol (Praha)     Country:  Czech Republic    
Other Details:
Languages:  eng     Pagination:  103-10     Citation Subset:  IM    
Affiliation:
Department of Physiology, Morehouse School of Medicine, Atlanta, Georgia 30310, USA. gleitch@msm.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cercopithecus aethiops
Epithelial Cells / microbiology
Giant Cells / microbiology*,  ultrastructure*
Immunohistochemistry
Microscopy, Electron, Scanning
Microsporidia / ultrastructure*
Microtubule-Organizing Center / microbiology
Patch-Clamp Techniques
Temperature
Grant Support
ID/Acronym/Agency:
R21 DK064573-01A1/DK/NIDDK NIH HHS; RR03034/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Microgemma vivaresi (Microsporidia: Tetramicridae): host reaction to xenomas induced in sea scorpion...
Next Document:  Role of the posterior vacuole in Spraguea lophii (Microsporidia) spore hatching.