| Multimodality imaging of T-cell hybridoma trafficking in collagen-induced arthritic mice: image-based estimation of the number of cells accumulating in mouse paws. | |
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MedLine Citation:
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PMID: 18163841 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Appropriate targeting of therapeutic cells is essential in adoptive cellular gene therapy (ACGT). Imaging cell trafficking in animal models and patients will guide development of ACGT protocols. Collagen type II (C-II)-specific T cell hybridomas are transduced with a lentivirus carrying a triple fusion reporter gene (TFR) construct consisting of a fluorescent reporter gene (RG), a bioluminescent RG (hRluc), and a positron emission tomography (PET) RG. Collagen-induced arthritic (CIA) mice are scanned with a bioluminescence imaging camera before and after implantation of various known cell quantities in their paws. Linear regression analysis yields equations relating two parameters of image signal intensity in mice paws to the quantity of hRluc expressing cells in the paws. Afterward, trafficking of intravenously injected cells is studied by quantitative analysis of bioluminescence images. Comparison of the average cell numbers does not demonstrate consistently higher accumulation of T-cell hybridomas in the paws with higher inflammation scores, and injecting more cells does not cause increased accumulation. MicroPET images illustrate above background signal in the inflamed paws and chest areas of CIA mice. The procedures described in this study can be used to derive equations for cells expressing other bioluminescent RGs and in other animal models. |
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Authors:
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Shahriar S Yaghoubi; Remi J Creusot; Pritha Ray; C Garrison Fathman; Sanjiv Sam Gambhir |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical optics Volume: 12 ISSN: 1083-3668 ISO Abbreviation: J Biomed Opt Publication Date: 2007 Nov-Dec |
Date Detail:
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Created Date: 2007-12-31 Completed Date: 2008-03-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: United States |
Other Details:
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Languages: eng Pagination: 064025 Citation Subset: IM |
Affiliation:
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Stanford University, Department of Radiology, Bio-X, Molecular Imaging Program, Stanford, California 94305-5427, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arthritis, Experimental / immunology*, pathology, therapy Collagen Type II / immunology Gene Therapy Genes, Reporter Hybridomas / immunology*, pathology Luminescent Measurements Lymphocyte Count Mice Mice, Inbred DBA Positron-Emission Tomography T-Lymphocytes / immunology*, pathology |
| Grant Support | |
ID/Acronym/Agency:
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2 P01 AI 36535-09/AI/NIAID NIH HHS; P50 CA114747/CA/NCI NIH HHS; P50 CA86306/CA/NCI NIH HHS; R01 CA82214-01/CA/NCI NIH HHS; R24 CA92865/CA/NCI NIH HHS; R33 AI55468-03/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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