Document Detail

Multimodality imaging of T-cell hybridoma trafficking in collagen-induced arthritic mice: image-based estimation of the number of cells accumulating in mouse paws.
MedLine Citation:
PMID:  18163841     Owner:  NLM     Status:  MEDLINE    
Appropriate targeting of therapeutic cells is essential in adoptive cellular gene therapy (ACGT). Imaging cell trafficking in animal models and patients will guide development of ACGT protocols. Collagen type II (C-II)-specific T cell hybridomas are transduced with a lentivirus carrying a triple fusion reporter gene (TFR) construct consisting of a fluorescent reporter gene (RG), a bioluminescent RG (hRluc), and a positron emission tomography (PET) RG. Collagen-induced arthritic (CIA) mice are scanned with a bioluminescence imaging camera before and after implantation of various known cell quantities in their paws. Linear regression analysis yields equations relating two parameters of image signal intensity in mice paws to the quantity of hRluc expressing cells in the paws. Afterward, trafficking of intravenously injected cells is studied by quantitative analysis of bioluminescence images. Comparison of the average cell numbers does not demonstrate consistently higher accumulation of T-cell hybridomas in the paws with higher inflammation scores, and injecting more cells does not cause increased accumulation. MicroPET images illustrate above background signal in the inflamed paws and chest areas of CIA mice. The procedures described in this study can be used to derive equations for cells expressing other bioluminescent RGs and in other animal models.
Shahriar S Yaghoubi; Remi J Creusot; Pritha Ray; C Garrison Fathman; Sanjiv Sam Gambhir
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  12     ISSN:  1083-3668     ISO Abbreviation:  J Biomed Opt     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2007-12-31     Completed Date:  2008-03-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  064025     Citation Subset:  IM    
Stanford University, Department of Radiology, Bio-X, Molecular Imaging Program, Stanford, California 94305-5427, USA.
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MeSH Terms
Arthritis, Experimental / immunology*,  pathology,  therapy
Collagen Type II / immunology
Gene Therapy
Genes, Reporter
Hybridomas / immunology*,  pathology
Luminescent Measurements
Lymphocyte Count
Mice, Inbred DBA
Positron-Emission Tomography
T-Lymphocytes / immunology*,  pathology
Grant Support
2 P01 AI 36535-09/AI/NIAID NIH HHS; P50 CA114747/CA/NCI NIH HHS; P50 CA86306/CA/NCI NIH HHS; R01 CA82214-01/CA/NCI NIH HHS; R24 CA92865/CA/NCI NIH HHS; R33 AI55468-03/AI/NIAID NIH HHS
Reg. No./Substance:
0/Collagen Type II

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