Document Detail


Multimodal silica-shelled quantum dots: direct intracellular delivery, photosensitization, toxic, and microcirculation effects.
MedLine Citation:
PMID:  18494515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present study, we describe a multimodal QD probe with combined fluorescent and paramagnetic properties, based on silica-shelled single QD micelles with incorporated paramagnetic substances [tris(2,2,6,6-tetramethyl-3,5-heptanedionate)/gadolinium] into the micelle and/or silica coat. The probe was characterized with high photoluminescence quantum yield and good positive MRI contrast, low cytotoxicity, and easy intracellular delivery in viable cells. The intravenous administration of the probe in experimental animals did not affect significantly the physiological parameters and microcirculation (e.g., heart rate, blood pressure, diameter and shape of blood vessels), which makes it appropriate for tracing of blood circulation and in vivo multimodal imaging using fluorescent confocal microscopy, two-photon microscopy, and MRI.
Authors:
Rumiana Bakalova; Zhivko Zhelev; Ichio Aoki; Kazuto Masamoto; Milka Mileva; Takayuki Obata; Makoto Higuchi; Veselina Gadjeva; Iwao Kanno
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Publication Detail:
Type:  Journal Article     Date:  2008-05-22
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  19     ISSN:  1520-4812     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-19     Completed Date:  2008-07-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1135-42     Citation Subset:  IM    
Affiliation:
Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263-8555, Japan. bakalova@nirs.go.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / diagnostic use,  metabolism,  toxicity
Blood Pressure / drug effects
Brain / blood supply*,  drug effects*
Contrast Media / metabolism,  toxicity
Fluorescent Dyes / diagnostic use,  metabolism,  toxicity
Gadolinium / chemistry
Heart Rate / drug effects
Hela Cells
Humans
Intracellular Space / metabolism*
Magnetics*
Micelles
Microcirculation / drug effects
Photosensitizing Agents / diagnostic use*,  metabolism*,  toxicity
Quantum Dots*
Rats
Silicon Dioxide / chemistry*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Contrast Media; 0/Fluorescent Dyes; 0/Micelles; 0/Photosensitizing Agents; 7440-54-2/Gadolinium; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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