|Multimodal cardiovascular magnetic resonance quantifies regional variation in vascular structure and function in patients with coronary artery disease: relationships with coronary disease severity.|
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|PMID: 22017860 Owner: NLM Status: MEDLINE|
|BACKGROUND: Cardiovascular magnetic resonance (CMR) of the vessel wall is highly reproducible and can evaluate both changes in plaque burden and composition. It can also measure aortic compliance and endothelial function in a single integrated examination. Previous studies have focused on patients with pre-identified carotid atheroma. We define these vascular parameters in patients presenting with coronary artery disease and test their relations to its extent and severity.
METHODS AND RESULTS: 100 patients with CAD [single-vessel (16%); two-vessel (39%); and three-vessel (42%) non-obstructed coronary arteries (3%)] were studied. CAD severity and extent was expressed as modified Gensini score (mean modified score 12.38 ± 5.3). A majority of carotid plaque was located in the carotid bulb (CB). Atherosclerosis in this most diseased segment correlated modestly with the severity and extent of CAD, as expressed by the modified Gensini score (R = 0.251, P < 0.05). Using the AHA plaque classification, atheroma class also associated with CAD severity (rho = 0.26, P < 0.05). The distal descending aorta contained the greatest plaque, which correlated with the degree of CAD (R = 0.222; P < 0.05), but with no correlation with the proximal descending aorta, which was relatively spared (R = 0.106; P = n. s.). Aortic distensibility varied along its length with the ascending aorta the least distensible segment. Brachial artery FMD was inversely correlated with modified Gensini score (R = -0.278; P < 0.05). In multivariate analysis, distal descending aorta atheroma burden, distensibility of the ascending aorta, carotid atheroma class and FMD were independent predictors of modified Gensini score.
CONCLUSIONS: Multimodal vascular CMR shows regional abnormalities of vascular structure and function that correlate modestly with the degree and extent of CAD.
|Ilias Kylintireas; Cheerag Shirodaria; Justin M S Lee; Colin Cunningon; Alistair Lindsay; Jane Francis; Matthew D Robson; Stefan Neubauer; Keith M Channon; Robin P Choudhury|
|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-10-21|
|Title: Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance Volume: 13 ISSN: 1532-429X ISO Abbreviation: J Cardiovasc Magn Reson Publication Date: 2011|
|Created Date: 2012-01-12 Completed Date: 2012-05-08 Revised Date: 2013-06-27|
Medline Journal Info:
|Nlm Unique ID: 9815616 Medline TA: J Cardiovasc Magn Reson Country: England|
|Languages: eng Pagination: 61 Citation Subset: IM|
|Department of Cardiovascular Medicine, University of Oxford and Oxford Centre for Clinical Magnetic Resonance Research, Oxford, UK.|
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Analysis of Variance
Aorta / pathology, physiopathology
Aortic Diseases / diagnosis*, pathology, physiopathology
Atherosclerosis / diagnosis*, pathology, physiopathology
Brachial Artery / pathology, physiopathology
Carotid Arteries / pathology
Carotid Artery Diseases / diagnosis*, pathology, physiopathology
Coronary Artery Disease / diagnosis*, pathology, physiopathology
Magnetic Resonance Imaging*
Predictive Value of Tests
Severity of Illness Index
|//British Heart Foundation; //Wellcome Trust|
Journal ID (nlm-ta): J Cardiovasc Magn Reson
Publisher: BioMed Central
Copyright ©2011 Kylintireas et al; licensee BioMed Central Ltd.
Received Day: 27 Month: 4 Year: 2011
Accepted Day: 21 Month: 10 Year: 2011
collection publication date: Year: 2011
Electronic publication date: Day: 21 Month: 10 Year: 2011
Volume: 13 Issue: 1
First Page: 61 Last Page: 61
Publisher Id: 1532-429X-13-61
PubMed Id: 22017860
|Multimodal cardiovascular magnetic resonance quantifies regional variation in vascular structure and function in patients with coronary artery disease: Relationships with coronary disease severity|
|Ilias Kylintireas1||Email: firstname.lastname@example.org|
|Cheerag Shirodaria1||Email: email@example.com|
|Justin MS Lee1||Email: firstname.lastname@example.org|
|Colin Cunningon1||Email: email@example.com|
|Alistair Lindsay1||Email: firstname.lastname@example.org|
|Jane Francis1||Email: email@example.com|
|Matthew D Robson1||Email: firstname.lastname@example.org|
|Stefan Neubauer1||Email: email@example.com|
|Keith M Channon1||Email: firstname.lastname@example.org|
|Robin P Choudhury1||Email: email@example.com|
1Department of Cardiovascular Medicine, University of Oxford and Oxford Centre for Clinical Magnetic Resonance Research (OCMR), Oxford, UK
Imaging biomarkers have proven useful in the evaluation of drugs used in the treatment of atherosclerosis [1,2]. A variety of invasive and non-invasive techniques have been applied to quantify plaque progression and regression,[3-9] including compositional , and metabolic changes . The common goal is to extract reliable and reproducible quantitative data that yield mechanistic insights in small numbers of patients and in a short time frame.
Coronary artery atheroma burden can be estimated in partially stenosed arteries with intravascular ultrasound (IVUS) [8,12-14]. The principal drawback of IVUS is its invasive nature, although serious complications (such as arterial dissection or acute vessel closure) are relatively rare (< 0.5%), the use of IVUS is effectively limited to the study of patients in whom coronary angiography is clinically indicated. Because significant stenoses are generally treated with balloon angioplasty and stents, IVUS is further confined to interrogation of non-stenotic segments (< 50%) of a different, single coronary artery.
B-mode (2-dimensional) ultrasound can quantify thickening of the intima and media of carotid arteries (CIMT), with high spatial resolution. CIMT is safe, non-invasive, reproducible, quick and cheap to perform and can be standardized for application in multiple centres  and has been widely used in atherosclerosis treatment trials [16-22] However, while providing quantitative data on wall thickness, CIMT is limited to the carotid arteries and does not provide useful information on composition or function of the vessel wall.
Cardiovascular magnetic resonance (CMR) is emerging as a useful complementary modality in the assessment of response to therapy in atherosclerosis [4,10,23,24]. Compared to existing approaches, CMR offers several distinct advantages. Firstly, unlike CIMT, CMR is a volumetric technique that is not confined to single-plane imaging of the carotid arteries, but can be used to interrogate volumes of the carotid arteries bilaterally, the aorta and the peripheral arteries. Unlike IVUS, CMR is non-invasive, and does not require ionising radiation for catheter positioning. CMR is highly reproducible and capable of evaluating changes in plaque volume in relatively small numbers of patients [25-27]. Furthermore, CMR offers an opportunity to measure not only plaque burden but also plaque composition [10,28-30] and to provide physiological assessments of vascular function such as pulse wave velocity, aortic compliance and endothelial function in the forearm in a single integrated examination [24,31-33].
Previous studies did not take into account the distribution of atheroma along the studied vessels and did not quantitatively relate this to CAD extent or severity on a per segment basis [34,35]. As CMR is poised to become more widely applied for the evaluation of cardiovascular drug therapies, it has become important to define the relationship between MR derived indices of peripheral vascular structure and function and coronary disease.
Here, we determine (1) the extent and distribution of atheroma burden in the carotid arteries and aorta, (2) the degree and distribution of aortic distensibility, (3) carotid artery composition, and (4) flow mediated vasodilatation of the forearm, all measured by CMR, in patients with CAD and relate these to the findings obtained from clinically-driven coronary angiography. This cohort is likely to be representative of the general coronary artery disease population that comprises the bulk of patients who will eventually receive atherosclerosis-modifying drugs.
Patients admitted for coronary angiography for investigation of chest pain or abnormal non-invasive cardiac investigations and who had also participated in a vascular CMR research protocol were eligible for inclusion. This approach was designed to capture a cohort that was representative of the spectrum of coronary disease severity. All component studies were approved by the Local Research Ethics Committee. Written informed consent was obtained from all patients. Patients with known chronic inflammatory conditions, infection, malignancy, and contraindications to CMR scanning were excluded.
Cholesterol and lipoprotein assays were performed using a Cobas-Mira Analyser (ABX Diagnostics, Shefford, UK). Total cholesterol was assayed using the enzymatic CHOD-PAP method and triglycerides were assayed using the enzymatic GPO-PAP method. HDL-cholesterol was assayed using a homogenous second generation PEGME method (Roche Diagnostics, Burgess Hill, UK).
X-Ray coronary arteriography was performed by the Judkins method. The severity (% stenosis) of coronary artery atherosclerosis was systematically estimated visually in each arterial segment, independently by two experienced observers, blinded to the clinical and CMR data. The coronary artery tree of each patient was scored using the modified Gensini method . In this scoring system, a cumulative numeric score is determined by the degree of luminal narrowing and the anatomical location of each stenosis. The modified Gensini score has been described and validated previously . The most severe stenosis in each of eight coronary segments was graded from 1 to 4 (1 = 1% to 49% lumen diameter reduction; 2 = 50% to 74% stenosis; 3 = 75% to 99% stenosis; 4 = 100% occlusion) to give a total score of between 0 and 32. This score therefore gives a measure that combines both the severity and extent of coronary atherosclerosis.
CMR was performed on a 1.5 Tesla Sonata system (Siemens, Erlangen) at a constant temperature and after at least 20 minutes' quiet rest. Blood pressure was monitored using a sphygmomanometer.
For assessment of atherosclerosis in the aorta, a stack of 11 transverse black blood turbo spin echo (TSE) images covering the descending thoracic aorta was acquired during diastole (sequence parameters: TR 1 R-R interval, TE 11 ms, in-plane resolution 0.8 mm, slice thickness 5 mm). For assessment of atherosclerosis in the carotid arteries, we obtained 11 black blood turbo spin echo (TSE) cross-sectional images of both arteries, centred 1cm below the lowest point of the bifurcation (sequence parameters: FOV 150 mm, TR 2 R-R intervals, TE 81 ms, resolution 0.5 mm × 0.5 mm in plane, slice thickness 3 mm) (Figure 1).
All images were assessed using an image quality scoring system that was based on a previously published approach . Images of quality score > 3 were analyzed. These images were segmented using semi-automated border detection algorithms developed using Matlab software (Mathworks Inc.) in order to define the inner (lumen) and outer vessel wall boundaries. Vessel wall area (WA) was calculated from the difference between these two contours, and also normalized to external vessel area to yield plaque index (PI), as previously described .
Mean carotid and aortic WA and PI were produced by averaging such measurements for the entire vessel (in the case of carotid indices the left and right vessels were combined to generate a mean value). To determine the distribution of atheroma along these vessels we produced segmental average WA and PI measurements for corresponding segments of the carotids [carotid bulb (CB), distal common carotid (DCC), and proximal common carotid (PCC)] and the aorta [proximal descending aorta (PDA), middle descending aorta (MDA) and distal descending aorta (DDA)].
High resolution, black blood, proton density (TR/TE: 1200/12), T1 (TR/TE: 700/12), and T2 (TR/TE: 3200/81) weighted turbo spin echo (TSE) and bright blood steady state free precession imaging was used to characterize carotid plaque composition(in plane resolution 0.5 mm × 0.5 mm, slice thickness 3 mm for all types of images). Images of each carotid vessel (covering 6 mm proximally and 6 mm distally to the carotid bulb) were surveyed for atherosclerosis of the common carotid, carotid bulb, carotid bifurcation and internal carotid artery. For each subject the most severe plaque was identified and allocated a rank based on the previously published Modified American Heart Association (AHA) Atherosclerotic Plaque Classification for CMR (ranging from 1-8).
ECG-gated, steady state free precession (SSFP) 'cine' images were acquired during breath-hold to determine aortic distensibility. The first was obtained at the level of the right pulmonary artery through the ascending and proximal descending aorta and the second through the distal aorta below the diaphragm (CMR parameters: repetition time (TR) 2.8 ms, echo time (TE) 1.4 ms, in-plane resolution 2 mm, slice thickness 7 mm, temporal resolution 40 ms). Maximum and minimum aortic cross-sectional areas over the cardiac cycle were determined using semi-automated edge detection algorithms developed using Matlab software (Mathworks Inc.) (25). Distensibility was calculated as the relative change in area divided by the pulse pressure. Aortic distensibility was assessed at 3 levels of the thoracic aorta: ascending (AA), proximal descending (PA) and distal descending (DA) aorta. Semi automated software was used to track the vessel boundary during the cardiac cycle and calculate the relative cross-sectional area change in order to estimate aortic distensibility.
Steady state free precession (SSFP) acquisitions were used to determine brachial artery reactivity, as previously described . SSFP Cross-sectional images of the brachial artery (typical parameters TR 6 ms, TE 3 ms, in-plane resolution 0.3 mm, slice thickness 3 mm) were acquired at baseline and following release of a cuff inflated to 50 mm Hg above systolic blood pressure on the forearm for five minutes. After a 10-minute interval, further brachial artery images were acquired following administration of 400 micrograms of sublingual glyceryl trinitrate (GTN). Post processing was performed using semi-automated edge detection methods developed with Matlab software (Mathworks Inc.). Maximum percentage change in brachial artery cross-sectional area at end-diastole was used to determine the response to each stimulus as previously described.
Statistical analyses were carried out using SPSS version 15 (SPSS Inc.). Normal distribution of data was confirmed using the Kolmogorov-Smirnov test. Not Normally distributed data were logarithmically transformed for analysis. Normally distributed data are presented as mean ± standard error while not normally distributed data are presented as median (25th, 75th percentile). One-way and repeated measures Analysis of Variance (ANOVA) with Bonferroni post hoc comparisons, Friedman's test, paired t tests and independent t test were used to compare numerical variables between groups as appropriate, while the chi-squared test was used for categorical variables. Pearson's coefficient was used for univariate correlation analysis. Stepwise multivariate regression analysis with backward elimination of independent variables was applied for the analysis of the association between CAD extent/severity and each of the MR derived vascular measures that had a significant univariate correlations with Gensini score. Independent variables were entered in the model if the level of significance of their correlation with the dependent variable was P < 0.05, while correlations were regarded as independent for variables retained in the model and reaching a P < 0.05 level of significance.
The study population comprised 100 patients who had undergone clinically-driven coronary angiography and who had participated in a vascular CMR research protocol (Table 1 for clinical characteristics). Accordingly, the population included patients with: single-vessel (16%); two-vessel (39%); and three-vessel (42%) coronary disease and non-obstructed coronary arteries (3%). The mean modified Gensini score was 12.38 ± 5.3 (range: 4 to 21).
In total, 2200 vessel MR images were obtained, of which 1520 were included in the analysis (69%). Vessels were excluded if fewer than 6 images met the inclusion threshold for analysis. This yielded 151 adequately imaged vessels (76 right and 77 left carotid arteries) out of the original 200. 83% of patients had at least 1 carotid artery amenable to analysis. The principal reason for exclusion was poor distinction between the outer vessel wall and the surrounding structures, rendering accurate wall area quantification impossible. The mean wall area was 29.8 mm2 and the mean plaque index 0.42 (± 0.004). Mean wall area, but not mean plaque index, was slightly higher in the right compared to the left carotid artery [RCA wall area = 31.1 (25.5 - 35.8) mm2 vs. LCA wall area = 29.6 (25.3 - 34.1; P < 0.05] [RCA plaque index = 0.41 (± 0.004) vs. LCA plaque index = 0.42 (± 0.005) mm2 , not significant]. Inter-observer variability between the operators for wall area was < 4%. Intra-observer variability was < 1% on the images accepted for analysis.
Taken as a whole, there was no correlation between the mean wall area of the carotid arteries and the extent of coronary atheroma indicated by the modified Gensini score (R = 0.177, P = n.s.).
Examination of the segmental distribution of atheroma along the carotid artery revealed that the majority of carotid plaque was located in the carotid bulb (CB) (Figure 2A and 2B). Mean carotid bulb wall area correlated with the severity and extent of CAD, as expressed by the modified Gensini score (R = 0.251, P < 0.05). There was no association between the modified Gensini score and wall area of the proximal and distal common carotid.
On the basis of our image quality system we included in the analysis patients with adequate quality (vessel image score ≥ 3) visualization of the common carotid, bifurcation and internal carotid of at least one of their vessels. 80/100 patients had adequate imaging of at least one vessel and 75/100 had 2 assessable carotid arteries (Additional file 1: flow chart 1). Maximal carotid atheroma class was: I-II (normal and near-normal circumferential thickening) for 11/80 (13.75%), III (focal thickening) for 34/80 (42.5%), IV-V (lipid core) for 20/80 (25%), VI (plaque hemorrhage and fibrous cap rupture) for 10/80 (12.5%), VII (calcified plaque) for 3/80 (3.75%), VIII (fibrotic plaque) for 2/80 (2.5%). Maximal carotid atheroma class was associated with CAD severity (rho = 0.26, P < 0.05) (Figure 3).
Vessels of which < 6 images were analysable were excluded, the principal reason for exclusion being poor distinction between the outer vessel wall and the surrounding structures. In total, 807 out of 1100 (73%) aortic cross-sectional images were included in the analysis. This corresponded to 96 interpretable studies out of 100 obtained (Additional file 1: flow chart 1). The mean wall area was 168.3 (150.5 - 193.9) mm2 and the mean plaque index was 0.3 (± 0.003). Inter-observer variability between the operators for wall area was < 3%. Intra-observer variability was < 1%. There was no significant correlation between mean aortic wall area and the severity of CAD (R = 0.198, P = n.s.).
When aortic segments were considered the middle and distal descending aorta slices contained the greatest volume of plaque in comparison with the proximal descending aorta (see Figure 2C, &2D). As for the carotid arteries, a significant correlation emerged between CAD severity and wall area measured in the more diseased segments of aorta. For the middle descending aorta (R = 0.208; P < 0.05) and for the distal descending aorta (R = 0.222; P < 0.05), but with no correlation with the proximal descending aorta, which was relatively spared (R = 0.106; P = n. s.).
Aortic distensibility varied progressively along its length (ascending aorta: 1.8 ± 0.12 × 10-3 mmHg-1 , proximal descending aorta: 2.5 ± 0.14 × 10-3 mmHg-1 , distal descending aorta: 3.4 ± 0.21 × 10-3 mmHg-1 ), with the ascending aorta being the least distensible segment (Figure 4).
Mean FMD in this population was 8.4 (0.7)%. Brachial artery FMD inversely correlated with modified Gensini score (R = -0.278; P < 0.05) indicating a quantitative relationship between the CAD severity and the degree of endothelial dysfunction (Figure 5).
Using multivariate regression analysis with inclusion of anthropometric (body mass index, waist hip ratio) and demographic data (age, gender), cardiovascular risk factors and MR indices as independent variables, as appropriate, none of the carotid plaque measurements and none of the classical risk factors emerged as independent predictors of modified Gensini score. Distal descending aorta atheroma burden, distensibility of the ascending aorta, maximal carotid atheroma class and FMD were the only independent predictors of modified Gensini score [β (SE) = 10.34 (0.08), P < 0.05, β (SE) = -7.29 (5.08), P < 0.005, β (SE) = 0.7(0.3), P < 0.05 and β (SE) = -0.19 (0.08), P < 0.05 respectively] (Table 2).
We report the multimodal vascular CMR characteristics of 100 patients that represent a spectrum of coronary artery severity. In this clinically relevant cohort, we identify regional variations in both (i) the distribution of atherosclerosis in the aorta and carotid arteries and (ii) aortic distensibility, measured at three sites. For both carotid arteries and aorta, plaque burden of only the most diseased segments correlated modestly with the extent and severity of coronary disease. We also detect, for the first time, a relationship between increasing carotid atheroma complexity, characterized by CMR, and CAD extent/severity. The AHA atheroma classification based on histological features reflects in part the sequential development of atheromatous lesions . On this basis, although plaques bearing features of vulnerability (i.e. IV-VI) are associated with higher risk of thromboembolism and cardiovascular events, higher class atheroma (i.e.VII-VIII) corresponds to more advanced atherosclerosis. The modest association that we detect between carotid atheroma classification and Gensini score is therefore pathophysiologically consistent with this theoretical scheme.
According to conventional approaches to MR determination of atheroma burden, per patient measurements are derived by averaging or summation across the full extent of imaged aorta and/or the combined carotid vessels [4,9]. However, the current study demonstrates a propensity for MR-determined plaque in the carotid bulb and the practice of averaging along the vessel may diminish artificially the true range of plaque burden and thereby reduce the power for quantitative comparisons. The significance of the regional variation in distribution is further reinforced by the relationship with coronary atherosclerosis, estimated using a modified version of the Gensini scoring system. By virtue of being determined by both (i) the degree of coronary stenoses and (ii) the number of stenosed segments, this score provides an insight in the extent of coronary disease. Univariate analysis revealed that atheroma burden of the common carotid did not correlate with CAD severity, while only a modest correlation with atheroma of the carotid bulb was detected. This result is consistent with previous reports of a selective relationship between ultrasonographic atheroma measurements at that level and angiographic coronary disease estimates . The correlation with angiographically-determined coronary disease was weak but comparable with that found in studies of ultrasound-measured IMT vs. quantitative coronary angiography . No more than a modest correlation is to be expected, since CMR measures within-wall plaque volume, while coronary angiography estimates plaque extent based on the two-dimensional projection of lesions that encroach into the vessel lumen, with very limited ability to estimate the true size of plaques. Furthermore, patients included in this study had symptomatic angina; biasing inclusion to the presence of at least one flow-limiting coronary stenosis, irrespective of total coronary plaque burden. A recent study measuring intima media thickness along several segments of the carotid using ultrasound has not only confirmed significant differences in atheroma accumulation between segments but also demonstrated a selectively stronger relationship between coronary atherosclerosis and carotid intima media measurements at the level of the carotid bulb/bifurcation . The importance of focusing segments of artery with most abundant atherosclerosis is further emphasized by the recent observation that larger wall areas show the greatest propensity for change (both regression and progression) over time . Furthermore, larger plaques may show greater compositional heterogeneity, presenting an opportunity to quantify both total plaque size and lipid rich core [10,42].
Previous CMR studies of carotid atherosclerosis have largely focused on patients selected for the presence of significant carotid disease [38,43]. Using such an approach, often accompanied by comparison of MR appearances with histological examination of explanted endarterectomy material, it has been possible to determine and validate the use of multi-contrast-CMR for the assessment of plaque size and composition. The current study extends this analysis to patients that are more representative of the general coronary artery disease population and defines their CMR-derived vascular characteristics.
Aortic distensibility, determined by CMR is diminished in smokers; type 2 diabetes; and obesity . In the Multi-Ethnic Study of Atherosclerosis, aortic distensibility at a single level was associated with increasing cardiovascular risk factors . Importantly, distensibility varies over a substantial 'dynamic range' and can show improvement in response to treatment in a short time frame [9,44]. The measurement of lumen cross-sectional area is readily obtained using automated, highly reproducible techniques . Here, we show marked regional differences in distensibility in patients with coronary artery disease. The reasons for diminished distensibility may not be simply explained. We and others have found that in some patients reduced distensibility is related to increased wall thickness, however this relationship does not hold in patients with diabetes where distensibility may be reduced to due compositional changes in the vessel wall, for instance due to cross linking of structural proteins by advanced glycation end products [32,47,48]. However, as we are normalizing distensibility measured at all three aortic sites to the same peripherally measured pulse pressure, the observed differences may not be directly related to vascular wall structure differences but rather to central pulse pressure differences along the length of the aorta. When testing the association with CAD severity we found that the relationship was only modest and limited to the ascending aorta.
Finally, the demonstrated lower variability of MR based FMD measurement in comparison to the corresponding ultrasonographic technique may account for detection of a modest, yet significant and independent, relationship between CAD severity and endothelial dysfunction in this study .
CMR confers the ability to obtain multiple complementary measures of vascular structure and function in an integrated examination. This ability will enable the characterization of patients with different stages of disease who are likely to manifest a spectrum of vascular structural and functional dysfunction and to respond differently to given interventions. There is potential benefit in knowing which vascular segments and vascular parameters assessable by MR should be prioritized for specific studies, especially as part of a time consuming protocol and relatively expensive technique. We suggest that these results can help define MR scanning priorities.
In patients with angiographic coronary artery disease, vascular CMR of the carotid arteries and aorta show atherosclerosis that is mostly apparent in the carotid bulb and distal descending aorta. Only the extent of plaque in these most diseased segments modestly correlated with the extent and severity of coronary disease. There were modest, yet independent, associations between CAD extent/severity and increasing carotid atheroma complexity, endothelial dysfunction and ascending aorta stiffening, as characterized by CMR. As CMR assumes greater importance for assessment of vascular structure and function, the parameters reported here will better inform the design of clinical trials.
The authors declare that they have no competing interests.
IK, CS and JMLcarried out the patient recruitment. IK, CS, JML, CC and JFcarried out the data acquisition. IK and CSperformed the image analysis. IK, CS, JML, CC and ALcontributed to the image reproducibility and quality analysis. RCP, CS and IKdeveloped the study hypothesis and concept. RCP, IK, SN and KMCinterpreted the results. IKperformed the statistical analysis. IK and RCPdrafted the manuscript. MDRdeveloped the MRI protocol and the image analysis software employed in this study. RCP, SN and KCMoffered scientific supervision and oversight for this project.
Flow chart 1- Vessel wall image quality. Flow chart highlighting the image quality criteria and demonstrating the image selection process for analysis of plaque burden (of the aorta and the carotids) and plaque composition (of the carotid arteries).
Click here for additional data file (1532-429X-13-61-S1.DOC)
RPC is a Wellcome Trust Senior Research Fellow in Clinical Science. Dr Ilias Kylintireas was supported financially by the State Scholarship foundation of Greece. This study was supported by the Oxford Comprehensive Biomedical Research Centre, NIHR funding scheme. The authors acknowledge the support of the BHF Centre of Research Excellence, Oxford.
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Clinical characteristics and risk factors.
|Age||64.17 (± 0.78)|
|Diabetes mellitus (1 or 2)||40/100|
|Total cholesterol||4.21 (± 0.09)|
|LDL||2.44 (± 0.09)|
|HDL||1.11 (± 0.03)|
|Triglycerides||1.87 (± 0.1)|
|Body mass index (BMI)||28.32 (± 0.41)|
|Calcium channel blockers||28/100|
|Oral hypoglycemic agents||26/100|
Independent Predictors of Gensini score.
|Predictors (R2 = 0.17)||β (SE)||P|
|Distal descending aorta wall area||10.34 (0.08)||< 0.05|
|Ascending Aorta distensibility||-7.29 (5.08)||< 0.005|
|FMD||-0.19 (0.08)||< 0.05|
|Maximal Carotid Atheroma Class||0.7 (0.3)||< 0.05|
Using multivariate regression analysis, Maximal Carotid Atheroma Class, distal descending aorta atheroma burden, distensibility of the ascending aorta and FMD were independent predictors of modified Gensini score.
Keywords: Atherosclerosis, magnetic resonance imaging, coronary artery disease.
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