Document Detail


Multilocus sequence typing (MLST) shows that the 'Iberian' clone of methicillin-resistant Staphylococcus aureus has spread to France and acquired reduced susceptibility to teicoplanin.
MedLine Citation:
PMID:  12205056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infection by methicillin-resistant Staphylococcus aureus (MRSA) presents a serious problem, as these organisms are resistant to a wide range of antibiotics. Moreover, MRSA with reduced susceptibility to glycopeptides (GISA) have emerged in recent years. In our hospital we were faced with an outbreak of GISA between January and March 2000, involving six patients who were free of MRSA when the GISA isolate emerged, and five of whom had not been given glycopeptides. The initial GISA isolate from the six patients was compared with six 'historical' multiple-resistant MRSA isolates (1996-1999), which had not been found to be GISA by the routinely used method. Antibiotic susceptibility was studied through the disc diffusion method, and MICs of glycopeptides were determined by Etest, agar and broth dilution techniques. Molecular strain typing was done by PFGE and multilocus sequence typing (MLST). All 12 isolates that belonged to the gentamicin-resistant group of MRSA were susceptible to vancomycin, but showed reduced susceptibility to teicoplanin through at least one MIC method. PFGE typing yielded five different but closely related profiles, and eight of the 12 clinical isolates were classified into a single PFGE type. MLST yielded an identical allelic profile for all 12 isolates, corresponding to the profile (3-3-1-12-4-4-16) of the 'Iberian' clone HPV107 of MRSA. MLST allowed us to confirm the presence of the 'Iberian' MRSA clone HPV107 with the same allelic profile in our hospital for several years. We can now note that strains of this clone have acquired reduced susceptibility to teicoplanin.
Authors:
B Heym; M Le Moal; L Armand-Lefevre; M-H Nicolas-Chanoine
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of antimicrobial chemotherapy     Volume:  50     ISSN:  0305-7453     ISO Abbreviation:  J. Antimicrob. Chemother.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-02     Completed Date:  2003-04-21     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7513617     Medline TA:  J Antimicrob Chemother     Country:  England    
Other Details:
Languages:  eng     Pagination:  323-9     Citation Subset:  IM    
Affiliation:
Microbiology Department, Hôpital Ambroise Paré, AP-HP and Faculté de Médecine Paris-Ouest, Université Paris V, 9 avenue Charles de Gaulle, 92100 Boulogne-Billancourt, France.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / therapeutic use*
Bacterial Typing Techniques
Cross Infection / microbiology
Disease Outbreaks
Drug Resistance, Multiple, Bacterial / genetics*
Electrophoresis, Gel, Pulsed-Field
France / epidemiology
Humans
Methicillin Resistance / genetics*
Microbial Sensitivity Tests
Staphylococcal Infections / drug therapy*,  epidemiology,  microbiology
Staphylococcus aureus / classification,  drug effects*,  genetics
Teicoplanin / therapeutic use*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 61036-62-2/Teicoplanin

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