Document Detail


Multilocus mutation scanning for the analysis of genetic variation within Malassezia (Basidiomycota: Malasseziales).
MedLine Citation:
PMID:  17514784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Members of the genus Malassezia are budding yeasts, characterized by a thick cell wall. Recently, these yeasts have received attention as emerging pathogens. They are common commensals on the skin of animals and can become pathogenic under the influence of various predisposing factors. Central to studying their taxonomy, systematics, and ecology and to diagnosis is the accurate identification of species or operational taxonomic units. To overcome the limitations of current phenotypic and biochemical methods of identification, a PCR-coupled SSCP approach, utilizing sequence variation (0.4-33.5%) in short regions (approximately 250-270 bp) of the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA and the chitin synthase-2 gene (chs-2), was assessed for the identification and differentiation of different species/genotypes of Malassezia, characterized previously by DNA sequencing. Genomic DNA samples (n = 30) from Malassezia isolates cultured from canine skin scrapings were assessed by SSCP analysis of the two different genetic loci, and unequivocal delineation between genotypes and species was achieved. This SSCP approach is considered to provide a practical tool for the rapid and reliable genetic characterization of Malassezia genotypes/species from dogs and for investigating their population genetics and ecology. It will also provide a powerful tool for studies of Malassezia isolates from other animal species.
Authors:
Claudia Cafarchia; Domenico Otranto; Bronwyn E Campbell; Maria Stefania Latrofa; Jacques Guillot; Robin B Gasser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  28     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-05-18     Completed Date:  2007-06-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1176-80     Citation Subset:  IM    
Affiliation:
Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria, Valenzano, Bari, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA, Fungal / analysis*
Dermatomycoses / veterinary
Dog Diseases / microbiology
Dogs
Electrophoresis, Agar Gel / methods*
Genetic Variation / genetics*
Malassezia / genetics*,  isolation & purification
Polymorphism, Single-Stranded Conformational
Skin / microbiology
Chemical
Reg. No./Substance:
0/DNA, Fungal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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