Document Detail


Multilineage potential of bone-marrow-derived mesenchymal stem cell cell sheets: implications for tissue engineering.
MedLine Citation:
PMID:  19951089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bone-marrow-derived mesenchymal stem cells (BMSCs) are a promising source of cells for tissue engineering due to their multilineage mesenchymal differentiation potential. Their ability to proliferate and differentiate into the osteogenic, chondrogenic, and adipogenic lineage makes them an attractive cell source as compared to the terminally differentiated cells. In tissue engineering, use of cell sheet technology is gaining popularity. It is based on culturing cells until hyperconfluence, and it has resulted in the reduction of the number of cells lost when seeding onto scaffolds. Thus, formation of cell sheets with multipotent cells, such as BMSCs, would be a promising alternative to the conventional method of cell seeding, that is, single-cell suspension. However, the multilineage potential of BMSC cell sheets has yet to be verified. Therefore, the aim of this study was to characterize the formation of a hyperconfluent BMSC cell sheet as well as the effects of the hyperconfluent culture conditions on the multipotentiality of BMSCs. Our results showed that the BMSC cell sheets remained viable. The cell sheets were rich with type I collagen and were shown to have retained their multipotentiality. Hence, the use of BMSC cell sheets for tissue engineering application seems promising.
Authors:
Eugene Yong-Shun See; Siew Lok Toh; James Cho Hong Goh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  16     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-06     Completed Date:  2010-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1421-31     Citation Subset:  IM    
Affiliation:
National University of Singapore, Singapore, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Adipogenesis / genetics
Animals
Base Sequence
Bone Marrow Cells / cytology*,  metabolism
Cell Culture Techniques / methods
Cell Differentiation
Cell Proliferation
Chondrogenesis / genetics
Coated Materials, Biocompatible
Core Binding Factor Alpha 1 Subunit / genetics
DNA Primers / genetics
Mesenchymal Stem Cells / cytology*,  metabolism
Multipotent Stem Cells / cytology*,  metabolism
Osteogenesis / genetics
PPAR gamma / genetics
RNA, Messenger / genetics,  metabolism
Rabbits
SOX9 Transcription Factor / genetics
Tissue Engineering / methods*
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Core Binding Factor Alpha 1 Subunit; 0/DNA Primers; 0/PPAR gamma; 0/RNA, Messenger; 0/SOX9 Transcription Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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