| Multilineage potential of bone-marrow-derived mesenchymal stem cell cell sheets: implications for tissue engineering. | |
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MedLine Citation:
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PMID: 19951089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone-marrow-derived mesenchymal stem cells (BMSCs) are a promising source of cells for tissue engineering due to their multilineage mesenchymal differentiation potential. Their ability to proliferate and differentiate into the osteogenic, chondrogenic, and adipogenic lineage makes them an attractive cell source as compared to the terminally differentiated cells. In tissue engineering, use of cell sheet technology is gaining popularity. It is based on culturing cells until hyperconfluence, and it has resulted in the reduction of the number of cells lost when seeding onto scaffolds. Thus, formation of cell sheets with multipotent cells, such as BMSCs, would be a promising alternative to the conventional method of cell seeding, that is, single-cell suspension. However, the multilineage potential of BMSC cell sheets has yet to be verified. Therefore, the aim of this study was to characterize the formation of a hyperconfluent BMSC cell sheet as well as the effects of the hyperconfluent culture conditions on the multipotentiality of BMSCs. Our results showed that the BMSC cell sheets remained viable. The cell sheets were rich with type I collagen and were shown to have retained their multipotentiality. Hence, the use of BMSC cell sheets for tissue engineering application seems promising. |
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Authors:
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Eugene Yong-Shun See; Siew Lok Toh; James Cho Hong Goh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Tissue engineering. Part A Volume: 16 ISSN: 1937-335X ISO Abbreviation: Tissue Eng Part A Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-06 Completed Date: 2010-07-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: United States |
Other Details:
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Languages: eng Pagination: 1421-31 Citation Subset: IM |
Affiliation:
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National University of Singapore, Singapore, Singapore. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipogenesis
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genetics Animals Base Sequence Bone Marrow Cells / cytology*, metabolism Cell Culture Techniques / methods Cell Differentiation Cell Proliferation Chondrogenesis / genetics Coated Materials, Biocompatible Core Binding Factor Alpha 1 Subunit / genetics DNA Primers / genetics Mesenchymal Stem Cells / cytology*, metabolism Multipotent Stem Cells / cytology*, metabolism Osteogenesis / genetics PPAR gamma / genetics RNA, Messenger / genetics, metabolism Rabbits SOX9 Transcription Factor / genetics Tissue Engineering / methods* |
| Chemical | |
Reg. No./Substance:
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0/Coated Materials, Biocompatible; 0/Core Binding Factor Alpha 1 Subunit; 0/DNA Primers; 0/PPAR gamma; 0/RNA, Messenger; 0/SOX9 Transcription Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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