| Multilineage co-culture of adipose-derived stem cells for tissue engineering. | |
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MedLine Citation:
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PMID: 23135884    Owner: NLM    Status: Publisher    |
Abstract/OtherAbstract:
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Stem cell interactions through paracrine cell signalling can regulate a range of cell responses, including metabolic activity, proliferation and differentiation. Moving towards the development of optimized tissue-engineering strategies with adipose-derived stem cells (ASCs), the focus of this study was on developing indirect co-culture models to study the effects of mature adipocytes, chondrocytes and osteoblasts on bovine ASC multilineage differentiation. For each lineage, ASC differentiation was characterized by histology, gene expression and protein expression, in the absence of key inductive differentiation factors for the ASCs. Co-culture with each of the mature cell populations was shown to successfully induce or enhance lineage-specific differentiation of the ASCs. In general, a more homogeneous but lower-level differentiation response was observed in co-culture as compared to stimulating the bovine ASCs with inductive differentiation media. To explore the role of the Wnt canonical and non-canonical signalling pathways within the model systems, the effects of the Wnt inhibitors WIF-1 and DKK-1 on multilineage differentiation in co-culture were assessed. The data indicated that Wnt signalling may play a role in mediating ASC differentiation in co-culture with the mature cell populations. Copyright © 2012 John Wiley & Sons, Ltd. |
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Authors:
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Yimu Zhao; Stephen D Waldman; Lauren E Flynn |
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Publication Detail:
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Type:Â JOURNAL ARTICLE Â Â Â Date:Â 2012-11-8 |
Journal Detail:
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Title: Journal of tissue engineering and regenerative medicine    Volume: -    ISSN: 1932-7005    ISO Abbreviation: J Tissue Eng Regen Med    Publication Date: 2012 Nov |
Date Detail:
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Created Date:Â 2012-11-8 Â Â Â Completed Date:Â - Â Â Â Revised Date:Â - Â Â Â |
Medline Journal Info:
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Nlm Unique ID: 101308490    Medline TA: J Tissue Eng Regen Med    Country: -    |
Other Details:
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Languages:Â ENG Â Â Â Pagination:Â - Â Â Â Citation Subset:Â - Â Â Â |
Copyright Information:
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Copyright © 2012 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Chemical Engineering, Queen's University, Kingston, ON, Canada; Human Mobility Research Centre, Kingston General Hospital, Kingston, ON, Canada. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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