| Multilayer nanoencapsulation. New approach for immune protection of human pancreatic islets. | |
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MedLine Citation:
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PMID: 16968004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immune protection of artificial tissue by means of pancreatic islet microencapsulation is a very ambitious new approach to avoid life-long immune suppression. But the success in the utilization of the alginate-beads with incorporated islets is unfortunately limited. Some of the problems cannot be solved by a two-component system, so polymer encapsulation of the microbeads was tested to improve the properties. In the present paper a pure nanoencapsulation multilayer approach was tested in order to reduce the size of the capsule and possibly apply in the future a multilayer capsule with individual properties in each layer or region of the capsule. Different polycations were attached in a self-assembly process. The advantage in using the surface charge of islets as binding site for the polyions is the guarantee of complete coverage after the second layer. Release of insulin was determined to characterize the function of the islets after encapsulation as well as the permeability of the capsule. Fluorescence microscopy was used to visualize the polyelectrolyte layers. Finally by means of an immune assay, the protection capability of the capsule was proved. In these first measurements the encapsulation with a multilayer nanocapsule was shown to be a possible alternative to the more space-consuming and random islet-trapping microencapsulation. |
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Authors:
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Silke Krol; Silvia del Guerra; Maria Grupillo; Alberto Diaspro; Alessandra Gliozzi; Piero Marchetti |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nano letters Volume: 6 ISSN: 1530-6984 ISO Abbreviation: Nano Lett. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-09-13 Completed Date: 2006-10-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101088070 Medline TA: Nano Lett Country: United States |
Other Details:
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Languages: eng Pagination: 1933-9 Citation Subset: IM |
Affiliation:
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Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy. krol@fisica.unige.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques
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methods* Cell Transplantation / instrumentation, methods* Cells, Cultured Coated Materials, Biocompatible / chemistry* Humans Islets of Langerhans / cytology*, immunology* Islets of Langerhans Transplantation / instrumentation, methods* Nanostructures / chemistry*, ultrastructure Tissue Engineering / methods |
| Chemical | |
Reg. No./Substance:
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0/Coated Materials, Biocompatible |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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