Document Detail


Multilayer nanoencapsulation. New approach for immune protection of human pancreatic islets.
MedLine Citation:
PMID:  16968004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immune protection of artificial tissue by means of pancreatic islet microencapsulation is a very ambitious new approach to avoid life-long immune suppression. But the success in the utilization of the alginate-beads with incorporated islets is unfortunately limited. Some of the problems cannot be solved by a two-component system, so polymer encapsulation of the microbeads was tested to improve the properties. In the present paper a pure nanoencapsulation multilayer approach was tested in order to reduce the size of the capsule and possibly apply in the future a multilayer capsule with individual properties in each layer or region of the capsule. Different polycations were attached in a self-assembly process. The advantage in using the surface charge of islets as binding site for the polyions is the guarantee of complete coverage after the second layer. Release of insulin was determined to characterize the function of the islets after encapsulation as well as the permeability of the capsule. Fluorescence microscopy was used to visualize the polyelectrolyte layers. Finally by means of an immune assay, the protection capability of the capsule was proved. In these first measurements the encapsulation with a multilayer nanocapsule was shown to be a possible alternative to the more space-consuming and random islet-trapping microencapsulation.
Authors:
Silke Krol; Silvia del Guerra; Maria Grupillo; Alberto Diaspro; Alessandra Gliozzi; Piero Marchetti
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nano letters     Volume:  6     ISSN:  1530-6984     ISO Abbreviation:  Nano Lett.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-13     Completed Date:  2006-10-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101088070     Medline TA:  Nano Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1933-9     Citation Subset:  IM    
Affiliation:
Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy. krol@fisica.unige.it
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques / methods*
Cell Transplantation / instrumentation,  methods*
Cells, Cultured
Coated Materials, Biocompatible / chemistry*
Humans
Islets of Langerhans / cytology*,  immunology*
Islets of Langerhans Transplantation / instrumentation,  methods*
Nanostructures / chemistry*,  ultrastructure
Tissue Engineering / methods
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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