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Multifunctional quantum-dot-based siRNA delivery for HPV18 E6 gene silence and intracellular imaging.
MedLine Citation:
PMID:  21784514     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The functional quantum dots (QDs) were specifically designed to overcome barriers in siRNA delivery such as siRNA protection, cellular penetration, endosomal release, carrier unpacking, intracellular transport and gene silencing. In this paper, two l-arginine-functional-modified CdSe/ZnSe QDs were synthesized as siRNA carriers to silence HPV18 E6 gene in HeLa cells. Using such constructs, these QDs showed significantly low cellular cytotoxicity and good siRNA protection. Flow cytometric and confocal microscopic analyses confirmed that the QDs delivered siRNA into HeLa cells efficiently. Importantly, superior gene silencing efficiency was achieved as evaluated by Reverse Transcription-PCR (RT-PCR) and Western blotting and HeLa cells growth was inhibited in xCELLigence installation analysis and MTT assay when treated with QD-siRNA complexes. Interestingly, the QDs coated with β-CD-l-Arg showed optimized property compared with those coated with l-Arg. Furthermore, these QDs complexes could also be used as nanocrystal probing agents, allowing real-time tracking and localization of QDs during delivery and transfection. The properties and capabilities of these QDs showed that amino acid-modified QDs could be used as useful siRNA carriers to effectively silence a target gene as well as fluorescence probes to analyze intracellular imaging in vivo.
Authors:
Jin-Ming Li; Mei-Xia Zhao; Hua Su; Yuan-Yuan Wang; Cai-Ping Tan; Liang-Nian Ji; Zong-Wan Mao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-22
Journal Detail:
Title:  Biomaterials     Volume:  -     ISSN:  1878-5905     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
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