| Multifunctional CD4⁺ T cells in patients with American cutaneous leishmaniasis. | |
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MedLine Citation:
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PMID: 22288594 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leishmaniasis is a group of important parasitic diseases affecting millions worldwide. To understand more clearly the quality of T helper type 1 (Th1) response stimulated after Leishmania infection, we applied a multiparametric flow cytometry protocol to evaluate multifunctional T cells induced by crude antigen extracts obtained from promastigotes of Leishmania braziliensis (LbAg) and Leishmania amazonensis (LaAg) in peripheral blood mononuclear cells from healed cutaneous leishmaniasis patients. Although no significant difference was detected in the percentage of total interferon (IFN)-γ-producing CD4(+) T cells induced by both antigens, multiparametric flow cytometry analysis revealed clear differences in the quality of Th1 responses. LbAg induced an important proportion of multifunctional CD4(+) T cells (28% of the total Th1 response evaluated), whereas LaAg induced predominantly single-positive cells (68%), and 57% of those were IFN-γ single-positives. Multifunctional CD4(+) T cells showed the highest mean fluorescence intensity (MFI) for the three Th1 cytokines assessed and MFIs for IFN-γ and interleukin-2 from those cells stimulated with LbAg were significantly higher than those obtained after LaAg stimulation. These major differences observed in the generation of multifunctional CD4(+) T cells suggest that the quality of the Th1 response induced by L. amazonensis antigens can be involved in the mechanisms responsible for the high susceptibility observed in L. amazonensis-infected individuals. Ultimately, our results call attention to the importance of studying a Th1 response regarding its quality, not just its magnitude, and indicate that this kind of evaluation might help understanding of the complex and diverse immunopathogenesis of American tegumentary leishmaniasis. |
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Authors:
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A B B Macedo; J C Sánchez-Arcila; A O Schubach; S C F Mendonça; A Marins-Dos-Santos; M de Fatima Madeira; T Gagini; M I F Pimentel; P M De Luca |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 167 ISSN: 1365-2249 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-01-31 Completed Date: 2012-03-22 Revised Date: 2013-04-15 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: England |
Other Details:
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Languages: eng Pagination: 505-13 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology. |
Affiliation:
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Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, Protozoan / administration & dosage CD4-Positive T-Lymphocytes / classification, immunology* Cytokines / biosynthesis Female Flow Cytometry Humans Interferon-gamma / biosynthesis Interleukin-2 / biosynthesis Leishmania braziliensis / immunology Leishmania mexicana / immunology Leishmaniasis, Cutaneous / immunology*, parasitology Male Middle Aged Species Specificity Th1 Cells / immunology Tumor Necrosis Factor-alpha / biosynthesis Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Protozoan; 0/Cytokines; 0/IL2 protein, human; 0/Interleukin-2; 0/Tumor Necrosis Factor-alpha; 82115-62-6/Interferon-gamma |
| Comments/Corrections | |
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