Document Detail


Multifrequency phase fluorescence study of hapten-antibody complexation.
MedLine Citation:
PMID:  2712301     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rotational dynamics of a phenytoin fluoroimmunoassay system is investigated by using multifrequency phase and modulation fluorescence spectroscopy. Results indicated that the fluorescein label on this relatively small hapten undergoes significant (greater than 45%) local motion even when bound to the phenytoin antibody. We attribute this local motion to unhindered rotation of the fluorescein moiety around the bond attaching it to phenytoin. This local motion affects the sensitivity of a steady-state polarization immunoassay dramatically.
Authors:
F V Bright
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  61     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-06-06     Completed Date:  1989-06-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  309-13     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies*
Fluorescence*
Haptens*
Mathematics
Grant Support
ID/Acronym/Agency:
S07 RR 07066/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Haptens
Comments/Corrections
Erratum In:
Anal Chem 1989 Jul 1;61(13):1472

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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