| Multifluorescence confocal microscopy: application for a quantitative analysis of hemostatic proteins in human venous valves. | |
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MedLine Citation:
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PMID: 23026998 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Confocal laser scanning microscopy is commonly used to visualize and quantify protein expression. Visualization of the expression of multiple proteins in the same region via multifluorescence allows for the analysis of differential protein expression. The defining step of multifluorescence labeling is the selection of primary antibodies from different host species. In addition, species-appropriate secondary antibodies must also be conjugated to different fluorophores so that each protein can be visualized in separate channels. Quantitative analysis of proteins labeled via multifluorescence can be used to compare relative changes in protein expression. Multifluoresecence labeling and analysis of fluorescence intensity within and among human venous specimens, for example, allowed us to determine that the anticoagulant phenotype of the venous valve is defined not by increased anticoagulant expression, but instead by significantly decreased procoagulant protein expression (Blood 114:1276-1279, 2009 and Histochem Cell Biol 135:141-152, 2011). |
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Authors:
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Winifred E Trotman; Douglas J Taatjes; Edwin G Bovill |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 931 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-10-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 85-95 Citation Subset: IM |
Affiliation:
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Department of Pathology, College of Medicine, University of Vermont, Burlington, VT, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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