Document Detail

Multielectrode mapping of slow-wave activity in the isolated rabbit duodenum.
MedLine Citation:
PMID:  8482690     Owner:  NLM     Status:  MEDLINE    
The technique of multiple simultaneous recordings from a large number of extracellular electrodes (> 100) is currently used in the study of normal and abnormal electrical conduction in the heart and the genesis of cardiac arrhythmias. To investigate whether such a system could also be applied in gastrointestinal electrophysiology, several studies were performed with this technique on segments of isolated rabbit duodenum. A multiple-electrode assembly consisting of 240 silver wires was positioned on the serosal surface of the duodenum, and the recorded signals were, after suitable processing, stored. Thereafter, analysis of all simultaneously recorded slow waves during a selected period of time was performed to reconstruct the pattern of conduction in the duodenum. The first results show that there is a considerable variation in conduction pattern, which is determined by the site of the natural pacemaker. Several experiments were performed to rule out possible deleterious effects of positioning the multiple-electrode assembly on the duodenum. Furthermore, prolonged periods of recording did not influence propagation speed and pattern provided that the positioning of the multiple electrode assembly was performed with care. Entrainment of the natural pacemaker was possible by applying electrical stimuli through 2 of the 240 extracellular electrodes during simultaneous recordings. In conclusion, multisite extracellular mapping of gastrointestinal smooth muscle is possible and can be used to study origin and spread of slow-wave activity.
W J Lammers; A al-Kais; S Singh; K Arafat; T Y el-Sharkawy
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  74     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-06-01     Completed Date:  1993-06-01     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1454-61     Citation Subset:  IM    
Department of Physiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain.
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MeSH Terms
Duodenum / physiology*
Electric Stimulation
Intestinal Mucosa / physiology
Muscle, Smooth / physiology*

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